Metarhodopsin III formation and decay kinetics: Comparison of bovine and human rhodopsin

J. W. Lewis, F. J G M Van Kuijk, J. A. Carruthers, D. S. Kliger

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Absorbance spectra were recorded at various times after photolysis of hypotonically washed membrane suspensions of human and bovine rhodopsin (pH 7 or 8, T = 20 or 37°C). Global data analysis showed that at 20°C, metarhodopsin III formation was the only process occurring from 1 to 30 min following photolysis. At pH 8, a significant amount of human metarhodopsin I was present before metarhodopsin III formed. At 37°C, two decay processes were seen, formation of metarhodopsin III (lifetime 113 ± 3 sec bovine, 93 ± 2 sec human) and formation of a species with the properties of n-retinylidine opsin (lifetime 900 ± 150 sec bovine, 2000 ± 350 sec human). A larger fraction of human rhodopsin (~ 50%) is converted to metarhodopsin III than is the case for bovine rhodopsin (~ 35%).

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalVision Research
Volume37
Issue number1
DOIs
StatePublished - Jan 1 1997

Keywords

  • Dark adaptation
  • Human rhodopsin
  • Metarhodopsin III
  • Photoregeneration
  • Retinol dehydrogenase

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