Metal ion catalysis during group II intron self-splicing: Parallels with the spliceosome

Erik J. Sontheimer, Peter M. Gordon, Joseph A. Piccirilli

Research output: Contribution to journalArticlepeer-review

107 Scopus citations

Abstract

The identical reaction pathway executed by the spliceosome and self- splicing group II intron ribozymes has prompted the idea that both may be derived from a common molecular ancestor. The minimal sequence and structural similarities between group II introns and the spliceosomal small nuclear RNAs, however, have left this proposal in question. Mechanistic comparisons between group II self-splicing introns and the spliceosome are therefore important in determining whether these two splicing machineries may be related. Here we show that 3'-sulfur substitution at the S' splice site of a group II intron causes a metal specificity switch during the first step of splicing. In contrast, 3'-sulfur substitution has no significant effect on the metal specificity of the second step of cis-splicing. Isolation of the second step uncovers a metal specificity switch that is masked during the cis-splicing reaction. These results demonstrate that group II intron ribozymes are metalloenzymes that use a catalytic metal ion for leaving group stabilization during both steps of self-splicing. Furthermore, because 3'- sulfur substitution of a spliceosomal intron has precisely the same effects as were observed during cis-splicing of the group II intron, these results provide striking parallels between the catalytic mechanisms employed by these two systems.

Original languageEnglish (US)
Pages (from-to)1729-1741
Number of pages13
JournalGenes and Development
Volume13
Issue number13
DOIs
StatePublished - Jul 1 1999

Keywords

  • 3'-S- phosphorothiolate
  • Group II intron
  • Metal ion catalysis
  • Phosphotransesterification
  • Ribozyme
  • Spliceosome

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