Metabolomic profiling of 17 bile acids in serum from patients with primary biliary cirrhosis and primary sclerosing cholangitis: A pilot study

Jocelyn Trottier, Andrzej Białek, Patrick Caron, Robert J. Straka, Jenny Heathcote, Piotr Milkiewicz, Olivier Barbier

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Background: Primary biliary cirrhosis and primary sclerosing cholangitis are two cholestatic diseases characterised by hepatic accumulation of bile acids. Aims: This study compares serum bile acid levels in patients with primary biliary cirrhosis and primary sclerosing cholangitis and from age and sex-matched non cholestatic donors. Methods: Seventeen bile acids were quantified using liquid chromatography coupled to tandem mass spectrometry. Serum samples from cholestatic patients were compared with those of non-cholestatic donors. Results: The concentration of total bile acids, taurine and glycine conjugates of primary bile acids was elevated in both patients with primary biliary cirrhosis and primary sclerosing cholangitis when compared to non-cholestatic donors. Samples from primary sclerosing cholangitis patients displayed reduced levels of secondary acids, when compared to non cholestatic and primary biliary cirrhosis sera. The ratio of total glycine versus total taurine conjugates was reduced in patients with primary biliary cirrhosis, but not in primary sclerosing cholangitis. Conclusion: The present study suggests that circulating bile acids are altered differentially in primary biliary cirrhosis and primary sclerosing cholangitis patients.

Original languageEnglish (US)
Pages (from-to)303-310
Number of pages8
JournalDigestive and Liver Disease
Volume44
Issue number4
DOIs
StatePublished - Apr 2012

Bibliographical note

Funding Information:
Grant support: This study was supported by grants from the Canadian Institute of Health Research (CIHR; grant #MOP-84338 ) and the Canadian Foundation for Innovation (CFI, grant #10469 ). J. Trottier is holder of a scholarship from CIHR. O. Barbier is holder of a salary grant from CIHR (New investigator award #MSH95330) and the “Fonds pour la Recherche en Santé du Québec, FRSQ” (junior II award). The GOLDN study was supported by the NIH Heart, Lung and Blood Institute Grant U 01 HL72524 , Genetic and Environmental Determinants of Triglycerides .

Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.

Keywords

  • Cholestasis
  • LC-MS/MS
  • Plasma bile acids
  • Primary biliary cirrhosis
  • Primary sclerosing cholangitis

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