Metabolomic investigation of methicillin-resistant staphylococcus aureus

Ting Lei, Lei Wang, Chi Chen, Yinduo Ji

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Metabolomics is becoming increasingly important as it provides a comprehensive analytical platform to better understand the biological functioning of a cell or organism. In recent years, microbial metabolomics has received much attention in research areas from new drug discovery to metabolic engineering. An efficient and accurate method to measure the intracellular metabolites of a specific microbial species is a key prerequisite for metabolome analysis. In this study, we describe a workflow focusing on the extraction and quantification of intracellular metabolites of Staphylococcus aureus. A filter-based bacteria sampling system was utilized to separate the media and bacteria; fast quenching with nitrogen was applied to prevent any metabolite leakage; a glass beads beater was used for intracellular metabolite extraction; and the LC-QTOF was combined to quantify the intracellular amino acids of S. aureus. This protocol is demonstrated to be an efficient method for analyzing the intracellular metabolites of S. aureus.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages251-258
Number of pages8
Volume1085
ISBN (Print)9781627036634
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1085
ISSN (Print)10643745

Keywords

  • Amino acids
  • LC-QTOF
  • Metabolome
  • MRSA
  • Staphylococcus aureus

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

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