Metabolism of pregnenolone by human breast cancer. Evidence for 17α-hydroxylase and 17,20-lyase

Y. J. Abul-Hajj; R. Iverson; D. T. Kiang

doi:10.1016/0039-128X(79)90094-1

Metabolism of pregnenolone by human breast cancer. Evidence for 17α-hydroxylase and 17,20-lyase

Y. J. Abul-Hajj, R. Iverson, D. T. Kiang

Medicinal Chemistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The metabolism of 7- ³H-pregnenolone was studied in vitro using 16 human breast carcinomas. All mammary tumors transformed pregnenolone to progesterone. All estrogen receptor poor tumors and 4 out of 8 estrogen receptor rich tumors converted pregnenolone to 17-hydroxypregnenolone. Five estrogen receptor poor tumors showed the presence of 17,20-lyase as evidenced by formation of dehydroepiandro-sterone and androstenedione. In two estrogen receptor poor tumors, conversions of pregnenolone to progesterone, 17-hydroxy pregnenolone, dehydroepiandrosterone androstenedione and finally to estradiol was documented, providing a hypothetical pathway for steroid metabolism in human breast cancer. The conversion of pregnenolone to 17-hydroxy-pregnenolone was significantly less in receptor rich tumors and was totally absent in 4 receptor rich tumors with estrogen receptors of over 45 fmol/mg protein.

Original language	English (US)
Pages (from-to)	817-827
Number of pages	11
Journal	Steroids
Volume	34
Issue number	7
DOIs	https://doi.org/10.1016/0039-128X(79)90094-1
State	Published - Dec 1979

Bibliographical note

Funding Information:
This investigation was supported in part Grants CA 20530 and CA22467 from the National the Graduate School, University of Minnesota

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

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title = "Metabolism of pregnenolone by human breast cancer. Evidence for 17α-hydroxylase and 17,20-lyase",

abstract = "The metabolism of 7- 3H-pregnenolone was studied in vitro using 16 human breast carcinomas. All mammary tumors transformed pregnenolone to progesterone. All estrogen receptor poor tumors and 4 out of 8 estrogen receptor rich tumors converted pregnenolone to 17-hydroxypregnenolone. Five estrogen receptor poor tumors showed the presence of 17,20-lyase as evidenced by formation of dehydroepiandro-sterone and androstenedione. In two estrogen receptor poor tumors, conversions of pregnenolone to progesterone, 17-hydroxy pregnenolone, dehydroepiandrosterone androstenedione and finally to estradiol was documented, providing a hypothetical pathway for steroid metabolism in human breast cancer. The conversion of pregnenolone to 17-hydroxy-pregnenolone was significantly less in receptor rich tumors and was totally absent in 4 receptor rich tumors with estrogen receptors of over 45 fmol/mg protein.",

author = "Abul-Hajj, {Y. J.} and R. Iverson and Kiang, {D. T.}",

note = "Funding Information: This investigation was supported in part Grants CA 20530 and CA22467 from the National the Graduate School, University of Minnesota Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1979",

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doi = "10.1016/0039-128X(79)90094-1",

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AU - Abul-Hajj, Y. J.

AU - Iverson, R.

AU - Kiang, D. T.

N1 - Funding Information: This investigation was supported in part Grants CA 20530 and CA22467 from the National the Graduate School, University of Minnesota Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1979/12

Y1 - 1979/12

N2 - The metabolism of 7- 3H-pregnenolone was studied in vitro using 16 human breast carcinomas. All mammary tumors transformed pregnenolone to progesterone. All estrogen receptor poor tumors and 4 out of 8 estrogen receptor rich tumors converted pregnenolone to 17-hydroxypregnenolone. Five estrogen receptor poor tumors showed the presence of 17,20-lyase as evidenced by formation of dehydroepiandro-sterone and androstenedione. In two estrogen receptor poor tumors, conversions of pregnenolone to progesterone, 17-hydroxy pregnenolone, dehydroepiandrosterone androstenedione and finally to estradiol was documented, providing a hypothetical pathway for steroid metabolism in human breast cancer. The conversion of pregnenolone to 17-hydroxy-pregnenolone was significantly less in receptor rich tumors and was totally absent in 4 receptor rich tumors with estrogen receptors of over 45 fmol/mg protein.

AB - The metabolism of 7- 3H-pregnenolone was studied in vitro using 16 human breast carcinomas. All mammary tumors transformed pregnenolone to progesterone. All estrogen receptor poor tumors and 4 out of 8 estrogen receptor rich tumors converted pregnenolone to 17-hydroxypregnenolone. Five estrogen receptor poor tumors showed the presence of 17,20-lyase as evidenced by formation of dehydroepiandro-sterone and androstenedione. In two estrogen receptor poor tumors, conversions of pregnenolone to progesterone, 17-hydroxy pregnenolone, dehydroepiandrosterone androstenedione and finally to estradiol was documented, providing a hypothetical pathway for steroid metabolism in human breast cancer. The conversion of pregnenolone to 17-hydroxy-pregnenolone was significantly less in receptor rich tumors and was totally absent in 4 receptor rich tumors with estrogen receptors of over 45 fmol/mg protein.

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Metabolism of pregnenolone by human breast cancer. Evidence for 17α-hydroxylase and 17,20-lyase

Abstract

Bibliographical note

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