Metabolism of [D10]phenanthrene to tetraols in smokers for potential lung cancer susceptibility assessment: Comparison of oral and inhalation routes of administration

Yan Zhong, Jing Wang, Steven G. Carmella, J. Bradley Hochalter, Diane Rauch, Andrew Oliver, Joni Jensen, Dorothy K. Hatsukami, Pramod Upadhyaya, Cheryl Zimmerman, Stephen S. Hecht

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are believed to be among the causative agents for lung cancer in smokers. PAHs require metabolic activation for carcinogenicity. One pathway produces diol epoxides that react with DNA, causing mutations. Because diol epoxides are converted to tetraols, quantitation of tetraols can potentially be used to identify smokers who may be at higher risk for lung cancer. Our approach uses [D10]phenanthrene, a labeled version of phenanthrene, a noncarcinogenic PAH structurally analogous to carcinogenic PAH. Although smokers are exposed to PAH by inhalation, oral dosing would be more practical for phenotyping studies. Therefore, we investigated [D10]phenanthrene metabolism in smokers after administration by inhalation in cigarette smoke or orally. Sixteen smokers received 10 μg of [D10]phenanthrene in a cigarette or orally. Plasma and urine samples were analyzed for [D10]r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4- tetrahydrophenanthrene ([D10]PheT), the major end product of the diol epoxide pathway, by gas chromatography-negative ion chemical ionization-tandem mass spectrometry. The ratios of [D10]PheT (oral dosing/inhalation) in 15 smokers were 1.03 ± 0.32 and 1.02 ± 0.35, based on plasma area under the concentrationtime curve (0-∞) and total 48-h urinary excretion, respectively. Overall, there was no significant difference in the extent of [D10]PheT formation after the two different routes of exposure in smokers. A large interindividual variation in [D10]PheT formation was observed. These results demonstrate that the level of [D 10]PheT in urine after oral dosing of [D10]phenanthrene can be used to assess individual capacity of PAH metabolism by the diol epoxide pathway.

Original languageEnglish (US)
Pages (from-to)353-361
Number of pages9
JournalJournal of Pharmacology and Experimental Therapeutics
Volume338
Issue number1
DOIs
StatePublished - Jul 2011

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