Abstract
Introduction: Swine dysentery caused by Brachyspira hyodysenteriae is a production limiting disease in pig farming. Currently antimicrobial therapy is the only treatment and control method available. Objective: The aim of this study was to characterize the metabolic response of porcine colon explants to infection by B. hyodysenteriae. Methods: Porcine colon explants exposed to B. hyodysenteriae were analyzed for histopathological, metabolic and pro-inflammatory gene expression changes. Results: Significant epithelial necrosis, increased levels of l-citrulline and IL-1α were observed on explants infected with B. hyodysenteriae. Conclusions: The spirochete induces necrosis in vitro likely through an inflammatory process mediated by IL-1α and NO.
Original language | English (US) |
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Article number | 83 |
Journal | Metabolomics |
Volume | 13 |
Issue number | 7 |
DOIs | |
State | Published - Jul 1 2017 |
Externally published | Yes |
Bibliographical note
Funding Information:This work was funded by an Utrecht University, Department of Farm Animal Health internal research fund (MC) and a NWO Gravitation project Institute for Chemical Immunology (CB).
Publisher Copyright:
© 2017, The Author(s).
Keywords
- Brachyspira
- Host-pathogen interactions
- In vitro organ culture
- Nitric oxide
- Pathophysiology
- Swine dysentery