Metabolic reprogramming augments potency of human pSTAT3-inhibited iTregs to suppress alloreactivity

Kelly Walton, Mario R. Fernandez, Elizabeth M. Sagatys, Jordan Reff, Jongphil Kim, Marie Catherine Lee, John V. Kiluk, Jane Yuet Ching Hui, David McKenna, Meghan Hupp, Colleen Forster, Michael A. Linden, Nicholas J. Lawrence, Harshani R. Lawrence, Joseph Pidala, Steven Z. Pavletic, Bruce R. Blazar, Said M. Sebti, John L. Cleveland, Claudio AnasettiBrian C. Betts

Research output: Contribution to journalArticle


Immunosuppressive donor Tregs can prevent graft-versus-host disease (GVHD) or solid-organ allograft rejection. We previously demonstrated that inhibiting STAT3 phosphorylation (pSTAT3) augments FOXP3 expression, stabilizing induced Tregs (iTregs). Here we report that human pSTAT3-inhibited iTregs prevent human skin graft rejection and xenogeneic GVHD yet spare donor antileukemia immunity. pSTAT3-inhibited iTregs express increased levels of skin-homing cutaneous lymphocyte-associated antigen, immunosuppressive GARP and PD-1, and IL-9 that supports tolerizing mast cells. Further, pSTAT3-inhibited iTregs significantly reduced alloreactive conventional T cells, Th1, and Th17 cells implicated in GVHD and tissue rejection and impaired infiltration by pathogenic Th2 cells. Mechanistically, pSTAT3 inhibition of iTregs provoked a shift in metabolism from oxidative phosphorylation (OxPhos) to glycolysis and reduced electron transport chain activity. Strikingly, cotreatment with coenzyme Q10 restored OxPhos in pSTAT3-inhibited iTregs and augmented their suppressive potency. These findings support the rationale for clinically testing the safety and efficacy of metabolically tuned, human pSTAT3-inhibited iTregs to control alloreactive T cells.

Original languageEnglish (US)
Article numbere136437
JournalJCI Insight
Issue number9
StatePublished - May 7 2020

Fingerprint Dive into the research topics of 'Metabolic reprogramming augments potency of human pSTAT3-inhibited iTregs to suppress alloreactivity'. Together they form a unique fingerprint.

  • Cite this

    Walton, K., Fernandez, M. R., Sagatys, E. M., Reff, J., Kim, J., Lee, M. C., Kiluk, J. V., Hui, J. Y. C., McKenna, D., Hupp, M., Forster, C., Linden, M. A., Lawrence, N. J., Lawrence, H. R., Pidala, J., Pavletic, S. Z., Blazar, B. R., Sebti, S. M., Cleveland, J. L., ... Betts, B. C. (2020). Metabolic reprogramming augments potency of human pSTAT3-inhibited iTregs to suppress alloreactivity. JCI Insight, 5(9), [e136437].