Abstract
Background: Metabolomics, the study of small-molecule metabolites, has increased understanding of human metabolic diseases, but has not been used to study equine metabolic syndrome (EMS). Objectives: (1) To examine the serum metabolome of Welsh Ponies with and without insulin dysregulation before and during an oral sugar test (OST). (2) To identify differences in metabolites in ponies with insulin dysregulation, obesity, or history of laminitis. Animals: Twenty Welsh Ponies (mean ± SD; 13.8 ± 9.0 years) classified as non-insulin dysregulated [CON] (n = 10, insulin < 30 mU/L) or insulin dysregulated [ID] (n = 10, insulin > 60 mU/L) at 75 minutes after administration of Karo syrup, obese (n = 6) or nonobese (n = 14), and history of laminitis (n = 9) or no history of laminitis (n = 11). Methods: Case-control study. Metabolomic analysis was performed on serum obtained at 0 minutes (baseline) and 75 minutes during the OST. Data were analyzed with multivariable mixed linear models with significance set at P ≤.05. Results: Metabolomic analysis of 646 metabolites (506 known) detected significant metabolite differences. At baseline, 55 metabolites (insulin response), 91 metabolites (obesity status), and 136 metabolites (laminitis history) were different. At 75 minutes, 51 metabolites (insulin response), 102 metabolites (obesity status), and 124 metabolites (laminitis history) were different. Conclusions and Clinical Importance: Use of metabolomics could have diagnostic utility for early detection of EMS and provide new knowledge regarding the pathophysiology of metabolic perturbations associated with this condition that might lead to improved clinical management.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1215-1233 |
| Number of pages | 19 |
| Journal | Journal of veterinary internal medicine |
| Volume | 32 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 1 2018 |
Bibliographical note
Funding Information:The authors acknowledge Metabolon Inc, Dr Kirk Pappan, and Dr Robert Mohney. The research described in this manuscript was funded by the National Institutes of Health NIAMS, Morris Animal Foundation, University of Minnesota College of Veterinary Medicine, University of Minnesota Equine Center, and Michigan State University College of Veterinary Medicine. The research described in this manuscript was performed on client owned farms located in Virginia, Maryland, Mississippi, Arkansas, and California. Metabolomic analysis was performed at Metabolon Inc. Work previously presented in abstract form at the 2015 American College of Veterinary Internal Medicine Forum, Indianapolis, Indiana.
Funding Information:
The authors acknowledge Metabolon Inc, Dr Kirk Pappan, and Dr Robert Mohney. The research described in this manuscript was funded by the National Institutes of Health NIAMS, Morris Animal Foundation, University of Minnesota College of Veterinary Medicine, University of Minnesota Equine Center, and Michigan State University College of Veterinary Medicine. The research described in this manuscript was performed on client owned farms located in Virginia, Maryland, Mississippi, Arkansas, and California. Metabolo-mic analysis was performed at Metabolon Inc. Work previously presented in abstract form at the 2015 American College of Veterinary Internal Medicine Forum, Indianapolis, Indiana.
Publisher Copyright:
Copyright © 2018 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
Keywords
- equine metabolic syndrome
- horse
- hyperinsulinemia
- insulin resistance
- metabolomics
- oral sugar test
