Mesoscale Calcium (Ca2+) Imaging in Freely Behaving Mice

Suhasa Kodandaramaiah, Mathew L. Rynes, Daniel A. Surinach, Kapil Saxena, Zahra Navabi, Eunsong Ko, Malachi Lehmann

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Mesoscale imaging of calcium dynamics has emerged as a powerful technique to study the coordination of activity of multiple cortical regions simultaneously during awake behavior. While much of the work in the field has focused on studying cortical dynamics during head-fixed preparations, we have recently developed the mini-mScope—a head mounted widefield imaging microscope capable of imaging large swathes of the dorsal cortex of freely behaving mice. In this chapter, we guide the reader with methodological details to build and use the mini-mScope for widefield Ca2+ imaging in freely behaving mice performing complex behaviors such as spatial navigation.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages75-100
Number of pages26
DOIs
StatePublished - 2025

Publication series

NameNeuromethods
Volume214
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.

Keywords

  • Fluorescence microscopy
  • Naturalistic behaviors
  • Neuroscience
  • Spatial navigation
  • Wide-field calcium imaging

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