Mesoderm induction in Xenopus is a zygotic event regulated by maternal VegT via TGFβ growth factors

Matt Kofron, Teresa Demel, Jenny Xanthos, Jamie Lohr, Benjamin Sun, Hazel Sive, Shin Ichi Osada, Chris Wright, Chris Wylie, Janet Heasman

Research output: Contribution to journalArticlepeer-review

222 Scopus citations

Abstract

The maternal transcription factor VegT is important for establishing the primary germ layers in Xenopus. In previous work, we showed that the vegetal masses of embryos lacking maternal VegT do not produce mesoderm-inducing signals and that mesoderm formation in these embryos occurred ectopically, from the vegetal area rather than the equatorial zone of the blastula. Here we have increased the efficiency of the depletion of maternal VegT mRNA and have studied the effects on mesoderm formation. We find that maternal VegT is required for the formation of 90% of mesodermal tissue, as measured by the expression of mesodermal markers MyoD, cardiac actin, Xbra, Xwnt8 and alphaT4 globin. Furthermore, the transcription of FGFs and TGFβs, Xnr1, Xnr2, Xnr4 and derriere does not occur in VegT-depleted embryos. We test whether these growth factors may be endogenous factors in mesoderm induction, by studying their ability to rescue the phenotype of VegT-depleted embryos, when their expression is restricted to the vegetal mass. We find that Xnr1, Xnr2, Xnr4 and derriere mRNA all rescue mesoderm formation, as well as the formation of blastopores and the wild-type body axis. Derriere rescues trunk and tail while nr1, nr2 and nr4 rescue head, trunk and tail. We conclude that mesoderm induction in Xenopus depends on a maternal transcription factor regulating these zygotic growth factors.

Original languageEnglish (US)
Pages (from-to)5759-5770
Number of pages12
JournalDevelopment
Volume126
Issue number24
StatePublished - Dec 1999

Keywords

  • Growth factor
  • Induction
  • Mesoderm
  • VegT
  • Xenopus

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