Menstrual phase does not influence ventilatory responses to group III/IV afferent signaling in eumenorrheic young females

Emma J Lee, Kathryn Vera, Ninitha Asirvatham-Jeyaraj, Daniel Chantigian, Mia Larson, Manda Keller-Ross

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1 Scopus citations


Estrogen can reduce sympathetic activity, but its effects on minute ventilation (V E) with group III/IV afferent activation remain unclear. This study examined the influence of estrogen on V E during lower-extremity exercise with group III/IV activation. Females completed two identical visits in follicular and ovulatory menstrual phases. Nine participants (age 25 ± 4 years) performed three minutes of baseline steady-state cycle ergometry and then group III/IV afferents were further activated with proximal thigh cuffs inflated to 20, 60, and 100 mmHg (randomized) for two minutes and five minutes of cycling between each occlusion. Metaboreflex was isolated by post-exercise circulatory occlusion. Ventilation was measured continuously and rating of perceived exertion (RPE) was recorded for each stage. During rest and exercise, V E (p < 0.001) and tidal volume (V T) (p = 0.033) were higher in the follicular than ovulatory phase. Minute ventilation, V T, and respiratory rate (R R) with ergoreflex and metaboreflex activation were similar across phases. With cuff occlusion of 100 mmHg, V E increased from baseline by 26.3 ± 7.0 L/min in the follicular phase (p < 0.001) and by 25.3±7.7 L/min in the ovulatory phase (p < 0.001), with no difference between phases (p> 0.05); R R and V T increased similarly with occlusion, also with no phase differences. In eumenorrheic females, menstrual phase influences ventilation but not ventilatory responses to group III/IV isolation.

Original languageEnglish (US)
Article number103712
JournalRespiratory Physiology and Neurobiology
StatePublished - Oct 2021

Bibliographical note

Funding Information:
The authors were supported by the National Institutes of Health (NIH) K01 ( AG064038-01A1 ) (MKR) and a University of Minnesota Grant-in Aid (MKR & EL).

Publisher Copyright:
© 2021 Elsevier B.V.


  • Ergoreflex
  • Estrogen
  • Exercise
  • Metaboreflex
  • Ventilation

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't


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