Abstract
Coordinated interplay between membrane proteins and the lipid bilayer is required for such processes as transporter function and the entrance of enveloped viruses into host cells. In this study, three-dimensional cryo-electron microscopy density maps of mature and immature flaviviruses were analyzed to assess the curvature of the membrane leaflets and its relation to membrane-bound viral glycoproteins. The overall morphology of the viral membrane is determined by the icosahedral scaffold composed of envelope (E) and membrane (M) proteins through interaction of the proteins' stem-anchor regions with the membrane. In localized regions, small membrane areas exhibit convex, concave, flat or saddle-shaped surfaces that are constrained by the specific protein organization within each membrane leaflet. These results suggest that the organization of membrane proteins in small enveloped viruses mediate the formation of membrane curvature.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 86-94 |
| Number of pages | 9 |
| Journal | Journal of Structural Biology |
| Volume | 183 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 2013 |
Bibliographical note
Funding Information:We are grateful to Drs. Jue Chen and Rushika Perera for helpful comments. The work was supported by an NIH Program Project Grant ( AI055672 to R.J.K. and M.G.R.) and an NIH R21 grant ( R21AI079473 to W.Z.). The computational work of membrane curvatures was conducted at the Minnesota Supercomputing Institute, University of Minnesota.
Keywords
- 3D
- Cryo-EM
- Cryo-electron microscopy
- DENV
- Dengue virus
- Enveloped virus
- Flavivirus
- Membrane curvature
- WNV
- West Nile virus
