Medicago truncatula as a model for dicot cell wall development

Mesfin Tesfaye, S. Samuel Yang, Joann F.S. Lamb, Hans Joachim G. Jung, Deborah A Samac, Carroll P. Vance, John W. Gronwald, Kathryn A. Vandenbosch

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

We have initiated a genome-wide transcript profiling study using the model legume Medicago truncatula to identify putative genes related to cell wall biosynthesis and regulatory function in legumes. We used the GeneChip® Medicago Genome Array to compare transcript abundance in elongating versus postelongation stem internode segments of two M. truncatula accessions and two Medicago sativa (alfalfa) clones with contrasting stem cell wall concentration and composition. Hundreds of differentially expressed probe sets between elongating and postelongation stem segments showed similar patterns of gene expression in the model legume and cultivated alfalfa. Differentially expressed genes included genes with putative functions associated with primary and secondary cell wall biosynthesis and growth. Mining of public microarray data for coexpressed genes with two marker genes for secondary cell wall synthesis identified additional candidate secondary cell wall-related genes. Coexpressed genes included protein kinases, transcription factors, and unclassified groups that were not previously reported with secondary cell wall-associated genes. M. truncatula has been recognized as an excellent model plant for legume genomics. The stem tissue transcriptome analysis, described here, indicates that M. truncatula has utility as a model plant for cell wall genomics in legumes in general and shows excellent potential for translating gene discoveries to its close relative, cultivated alfalfa, in particular. The natural variation for stem cell wall traits in Medicago may offer a new tool to study an expanded repertoire of valuable agronomic traits in related species, including woody dicots in the eurosid I clade.

Original languageEnglish (US)
Pages (from-to)59-76
Number of pages18
JournalBioenergy Research
Volume2
Issue number1-2
DOIs
StatePublished - Jun 2009

Keywords

  • Alfalfa
  • Genomics
  • Medicago truncatula
  • Stems
  • Transcript analysis

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