Mediation of neurogenic ion transport by acetylcholine, prostanoids and 5-hydroxytryptamine in porcine ileum

DeWayne Townsend, Melissa A. Casey, David R Brown

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Enteric neural activity modulates active transepithelial ion transport in the intestine. We investigated the neural circuits mediating neurogenic secretion in mucosal explants from porcine ileum. Transmural electrical stimulation increased short-circuit current, a measure of active ion transport, by 35 ± 2 μA/cm2. The neuronal Na+ channel blocker saxitoxin, the muscarinic cholinergic receptor antagonist atropine, the 5-hydroxytryptamine3 receptor antagonist tropisetron, and the cyclooxygenase inhibitor indomethacin inhibited this response. In addition, tropisetron inhibited the atropine-resistant portion of the response, and both atropine and indomethacin attenuated the saxitoxin-resistant component. Neurogenic secretion in porcine ileum appears to be mediated by tryptaminergic and prostanoid-sensitive cholinergic pathways.

Original languageEnglish (US)
Pages (from-to)285-289
Number of pages5
JournalEuropean Journal of Pharmacology
Volume519
Issue number3
DOIs
StatePublished - Sep 20 2005

Bibliographical note

Funding Information:
This investigation was supported in part by National Institutes of Health grants R01 DA-10200 and T32 DA-07234.

Keywords

  • 5-hydroxytryptamine receptor
  • Intestinal mucosa
  • Muscarinic cholinergic receptor
  • Short-circuit current
  • Transmural electrical stimulation

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