Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids

Madhusudan V. Peshwa, Florence J. Wu, Harvey L. Sharp, Frank B. Cerra, Wei Shou Hu

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Freshly harvested rat hepatocytes form spheroids on uncoated positively charged polystyrene surfaces. Time lapse microscopy revealed that cell movement and reorganization were involved in spheroid formation. Ultrastructural evaluation using scanning and transmission electron microscopy indicated polarized cellular morphology and extensive cell-cell communication within spheroids. Bile canalicular structures were observed to surround each individual hepatocyte, forming an intricate three-dimensional continuous network of channels that appeared to end as pores/holes on the surface of the spheroid. The maintenance of differentiated cellular morphology coincided with preservation of hepatocyte viability and enhanced levels of tissue specific functions in spheroids.

Original languageEnglish (US)
Pages (from-to)197-203
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume32
Issue number4
DOIs
StatePublished - Apr 1996

Fingerprint

Hepatocytes
Scanning Transmission Electron Microscopy
Polystyrenes
Bile
Cell Communication
Cell Movement
Microscopy
Maintenance

Keywords

  • hepatocyte
  • multicellular aggregate
  • spheroid
  • time lapse microscopy

Cite this

Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids. / Peshwa, Madhusudan V.; Wu, Florence J.; Sharp, Harvey L.; Cerra, Frank B.; Hu, Wei Shou.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 32, No. 4, 04.1996, p. 197-203.

Research output: Contribution to journalArticle

Peshwa, Madhusudan V. ; Wu, Florence J. ; Sharp, Harvey L. ; Cerra, Frank B. ; Hu, Wei Shou. / Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids. In: In Vitro Cellular and Developmental Biology - Animal. 1996 ; Vol. 32, No. 4. pp. 197-203.
@article{9bc72a98120a4ed3a866411931015a3c,
title = "Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids",
abstract = "Freshly harvested rat hepatocytes form spheroids on uncoated positively charged polystyrene surfaces. Time lapse microscopy revealed that cell movement and reorganization were involved in spheroid formation. Ultrastructural evaluation using scanning and transmission electron microscopy indicated polarized cellular morphology and extensive cell-cell communication within spheroids. Bile canalicular structures were observed to surround each individual hepatocyte, forming an intricate three-dimensional continuous network of channels that appeared to end as pores/holes on the surface of the spheroid. The maintenance of differentiated cellular morphology coincided with preservation of hepatocyte viability and enhanced levels of tissue specific functions in spheroids.",
keywords = "hepatocyte, multicellular aggregate, spheroid, time lapse microscopy",
author = "Peshwa, {Madhusudan V.} and Wu, {Florence J.} and Sharp, {Harvey L.} and Cerra, {Frank B.} and Hu, {Wei Shou}",
year = "1996",
month = "4",
doi = "10.1007/BF02722946",
language = "English (US)",
volume = "32",
pages = "197--203",
journal = "In Vitro Cellular and Developmental Biology - Plant",
issn = "1071-2690",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids

AU - Peshwa, Madhusudan V.

AU - Wu, Florence J.

AU - Sharp, Harvey L.

AU - Cerra, Frank B.

AU - Hu, Wei Shou

PY - 1996/4

Y1 - 1996/4

N2 - Freshly harvested rat hepatocytes form spheroids on uncoated positively charged polystyrene surfaces. Time lapse microscopy revealed that cell movement and reorganization were involved in spheroid formation. Ultrastructural evaluation using scanning and transmission electron microscopy indicated polarized cellular morphology and extensive cell-cell communication within spheroids. Bile canalicular structures were observed to surround each individual hepatocyte, forming an intricate three-dimensional continuous network of channels that appeared to end as pores/holes on the surface of the spheroid. The maintenance of differentiated cellular morphology coincided with preservation of hepatocyte viability and enhanced levels of tissue specific functions in spheroids.

AB - Freshly harvested rat hepatocytes form spheroids on uncoated positively charged polystyrene surfaces. Time lapse microscopy revealed that cell movement and reorganization were involved in spheroid formation. Ultrastructural evaluation using scanning and transmission electron microscopy indicated polarized cellular morphology and extensive cell-cell communication within spheroids. Bile canalicular structures were observed to surround each individual hepatocyte, forming an intricate three-dimensional continuous network of channels that appeared to end as pores/holes on the surface of the spheroid. The maintenance of differentiated cellular morphology coincided with preservation of hepatocyte viability and enhanced levels of tissue specific functions in spheroids.

KW - hepatocyte

KW - multicellular aggregate

KW - spheroid

KW - time lapse microscopy

UR - http://www.scopus.com/inward/record.url?scp=0029945884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029945884&partnerID=8YFLogxK

U2 - 10.1007/BF02722946

DO - 10.1007/BF02722946

M3 - Article

C2 - 8727043

AN - SCOPUS:0029945884

VL - 32

SP - 197

EP - 203

JO - In Vitro Cellular and Developmental Biology - Plant

JF - In Vitro Cellular and Developmental Biology - Plant

SN - 1071-2690

IS - 4

ER -