Mechanistic issues of the interaction of the hairpin-forming domain of tBid with mitochondrial cardiolipin

François Gonzalvez, Fabrizio Pariselli, Olivier Jalmar, Pauline Dupaigne, Franck Sureau, Marc Dellinger, Eric A. Hendrickson, Sophie Bernard, Patrice X. Petit

Research output: Contribution to journalArticle

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Abstract

Background: The pro-apoptotic effector Bid induces mitochondrial apoptosis in synergy with Bax and Bak. In response to death receptors activation, Bid is cleaved by caspase-8 into its active form, tBid (truncated Bid), which then translocates to the mitochondria to trigger cytochrome c release and subsequent apoptosis. Accumulating evidence now indicate that the binding of tBid initiates an ordered sequences of events that prime mitochondria from the action of Bax and Bak: (1) tBid interacts with mitochondria via a specific binding to cardiolipin (CL) and immediately disturbs mitochondrial structure and function idependently of its BH3 domain; (2) Then, tBid activates through its BH3 domain Bax and/or Bak and induces their subsequent oligomerization in mitochondrial membranes. To date, the underlying mechanism responsible for targeting tBid to mitochondria and disrupting mitochondrial bioenergetics has yet be elucidated. Principal Findings:The present study investigates the mechanism by which tBid interacts with mitochondria issued from mouse hepatocytes and perturbs mitochondrial function. We show here that the helix αH6 is responsible for targeting tBid to mitochondrial CL and disrupting mitochondrial bioenergetics. In particular, αH6 interacts with mitochondria through electrostatic interactions involving the lysines 157 and 158 and induces an inhibition of state-3 respiration and an uncoupling of state-4 respiration. These changes may represent a key event that primes mitochondria for the action of Bax and Bak. In addition, we also demonstrate that tBid required its helix αH6 to efficiently induce cytochrome c release and apoptosis. Conclusions: Our findings provide new insights into the mechanism of action of tBid, and particularly emphasize the importance of the interaction of the helix αH6 with CL for both mitochondrial targeting and pro-apoptotic activity of tBid. These support the notion that tBid acts as a bifunctional molecule: first, it binds to mitochondrial CL via its helix αH6 and destabilizes mitochondrial structure and function, and then it promotes through its BH3 domain the activation and oligomerization of Bax and/or Bak, leading to cytochrome c release and execution of apoptosis. Our findings also imply an active role of the membrane in modulating the interactions between Bcl-2 proteins that has so far been underestimated.

Original languageEnglish (US)
Article numbere9342
JournalPloS one
Volume5
Issue number2
DOIs
StatePublished - Feb 22 2010

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Mitochondria
Cardiolipins
cardiolipins
mitochondria
cytochrome c
apoptosis
Cytochromes c
Apoptosis
Oligomerization
cell respiration
energy metabolism
Energy Metabolism
Respiration
Chemical activation
Membranes
caspase-8
Death Domain Receptors
electrostatic interactions
Caspase 8
Mitochondrial Membranes

Cite this

Gonzalvez, F., Pariselli, F., Jalmar, O., Dupaigne, P., Sureau, F., Dellinger, M., ... Petit, P. X. (2010). Mechanistic issues of the interaction of the hairpin-forming domain of tBid with mitochondrial cardiolipin. PloS one, 5(2), [e9342]. https://doi.org/10.1371/journal.pone.0009342

Mechanistic issues of the interaction of the hairpin-forming domain of tBid with mitochondrial cardiolipin. / Gonzalvez, François; Pariselli, Fabrizio; Jalmar, Olivier; Dupaigne, Pauline; Sureau, Franck; Dellinger, Marc; Hendrickson, Eric A.; Bernard, Sophie; Petit, Patrice X.

In: PloS one, Vol. 5, No. 2, e9342, 22.02.2010.

Research output: Contribution to journalArticle

Gonzalvez, F, Pariselli, F, Jalmar, O, Dupaigne, P, Sureau, F, Dellinger, M, Hendrickson, EA, Bernard, S & Petit, PX 2010, 'Mechanistic issues of the interaction of the hairpin-forming domain of tBid with mitochondrial cardiolipin', PloS one, vol. 5, no. 2, e9342. https://doi.org/10.1371/journal.pone.0009342
Gonzalvez F, Pariselli F, Jalmar O, Dupaigne P, Sureau F, Dellinger M et al. Mechanistic issues of the interaction of the hairpin-forming domain of tBid with mitochondrial cardiolipin. PloS one. 2010 Feb 22;5(2). e9342. https://doi.org/10.1371/journal.pone.0009342
Gonzalvez, François ; Pariselli, Fabrizio ; Jalmar, Olivier ; Dupaigne, Pauline ; Sureau, Franck ; Dellinger, Marc ; Hendrickson, Eric A. ; Bernard, Sophie ; Petit, Patrice X. / Mechanistic issues of the interaction of the hairpin-forming domain of tBid with mitochondrial cardiolipin. In: PloS one. 2010 ; Vol. 5, No. 2.
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AU - Sureau, Franck

AU - Dellinger, Marc

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N2 - Background: The pro-apoptotic effector Bid induces mitochondrial apoptosis in synergy with Bax and Bak. In response to death receptors activation, Bid is cleaved by caspase-8 into its active form, tBid (truncated Bid), which then translocates to the mitochondria to trigger cytochrome c release and subsequent apoptosis. Accumulating evidence now indicate that the binding of tBid initiates an ordered sequences of events that prime mitochondria from the action of Bax and Bak: (1) tBid interacts with mitochondria via a specific binding to cardiolipin (CL) and immediately disturbs mitochondrial structure and function idependently of its BH3 domain; (2) Then, tBid activates through its BH3 domain Bax and/or Bak and induces their subsequent oligomerization in mitochondrial membranes. To date, the underlying mechanism responsible for targeting tBid to mitochondria and disrupting mitochondrial bioenergetics has yet be elucidated. Principal Findings:The present study investigates the mechanism by which tBid interacts with mitochondria issued from mouse hepatocytes and perturbs mitochondrial function. We show here that the helix αH6 is responsible for targeting tBid to mitochondrial CL and disrupting mitochondrial bioenergetics. In particular, αH6 interacts with mitochondria through electrostatic interactions involving the lysines 157 and 158 and induces an inhibition of state-3 respiration and an uncoupling of state-4 respiration. These changes may represent a key event that primes mitochondria for the action of Bax and Bak. In addition, we also demonstrate that tBid required its helix αH6 to efficiently induce cytochrome c release and apoptosis. Conclusions: Our findings provide new insights into the mechanism of action of tBid, and particularly emphasize the importance of the interaction of the helix αH6 with CL for both mitochondrial targeting and pro-apoptotic activity of tBid. These support the notion that tBid acts as a bifunctional molecule: first, it binds to mitochondrial CL via its helix αH6 and destabilizes mitochondrial structure and function, and then it promotes through its BH3 domain the activation and oligomerization of Bax and/or Bak, leading to cytochrome c release and execution of apoptosis. Our findings also imply an active role of the membrane in modulating the interactions between Bcl-2 proteins that has so far been underestimated.

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