Mechanisms underlying Mannheimia haemolytica leukotoxin-induced oncosis and apoptosis of bovine alveolar macrophages

Praveen Thumbikat; Dileepan T; Mathur S Kannan; Samuel K. Maheswaran

doi:10.1016/j.micpath.2005.01.002

Mechanisms underlying Mannheimia haemolytica leukotoxin-induced oncosis and apoptosis of bovine alveolar macrophages

Praveen Thumbikat, Dileepan T, Mathur S Kannan, Samuel K. Maheswaran

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41 Scopus citations

Abstract

Mannheimia (Pasteurella) haemolytica leukotoxin (LktA) binds to the bovine β2 integrins (such as LFA-1 - CD11a/CD18) and leads to subsequent cellular effects in a dose dependent manner. The objectives of this study were to delineate the mechanisms that underlie LktA-induced oncosis and apoptosis and to examine the role of LktA/LFA-1 interaction in these events. The results demonstrate that LktA-induced oncosis proceeds through a LFA-1 and caspase-1 dependent pathway referred to as 'pyrotosis', as well as through a LFA-1- and caspase-1-independent pathway. LktA-induced apoptosis in alveolar macrophages involves activation of caspase-3 and engages the extrinsic and intrinsic pathways of apoptosis, with the extrinsic pathway being dependent on LFA-1 signaling and TNFα.

Original language	English (US)
Pages (from-to)	161-172
Number of pages	12
Journal	Microbial Pathogenesis
Volume	38
Issue number	4
DOIs	https://doi.org/10.1016/j.micpath.2005.01.002
State	Published - Apr 2005

Bibliographical note

Funding Information:
This study was supported by funds from USDA-NRICG (2000–35204–9230) and the Minnesota Agricultural Experiment Station. We thank Charles J. Czuprynski from the Department of Pathobiological Sciences, University of Wisconsin for helpful discussions.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

Apoptosis
Caspase-8
Mannheimia haemolytica leukotoxin
Oncosis
Pore formation
Pyrotosis

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10.1016/j.micpath.2005.01.002

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title = "Mechanisms underlying Mannheimia haemolytica leukotoxin-induced oncosis and apoptosis of bovine alveolar macrophages",

abstract = "Mannheimia (Pasteurella) haemolytica leukotoxin (LktA) binds to the bovine β2 integrins (such as LFA-1 - CD11a/CD18) and leads to subsequent cellular effects in a dose dependent manner. The objectives of this study were to delineate the mechanisms that underlie LktA-induced oncosis and apoptosis and to examine the role of LktA/LFA-1 interaction in these events. The results demonstrate that LktA-induced oncosis proceeds through a LFA-1 and caspase-1 dependent pathway referred to as 'pyrotosis', as well as through a LFA-1- and caspase-1-independent pathway. LktA-induced apoptosis in alveolar macrophages involves activation of caspase-3 and engages the extrinsic and intrinsic pathways of apoptosis, with the extrinsic pathway being dependent on LFA-1 signaling and TNFα.",

keywords = "Apoptosis, Caspase-8, Mannheimia haemolytica leukotoxin, Oncosis, Pore formation, Pyrotosis",

author = "Praveen Thumbikat and Dileepan T and Kannan, {Mathur S} and Maheswaran, {Samuel K.}",

note = "Funding Information: This study was supported by funds from USDA-NRICG (2000–35204–9230) and the Minnesota Agricultural Experiment Station. We thank Charles J. Czuprynski from the Department of Pathobiological Sciences, University of Wisconsin for helpful discussions. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2005",

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doi = "10.1016/j.micpath.2005.01.002",

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AU - Thumbikat, Praveen

AU - T, Dileepan

AU - Kannan, Mathur S

AU - Maheswaran, Samuel K.

N1 - Funding Information: This study was supported by funds from USDA-NRICG (2000–35204–9230) and the Minnesota Agricultural Experiment Station. We thank Charles J. Czuprynski from the Department of Pathobiological Sciences, University of Wisconsin for helpful discussions. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2005/4

Y1 - 2005/4

N2 - Mannheimia (Pasteurella) haemolytica leukotoxin (LktA) binds to the bovine β2 integrins (such as LFA-1 - CD11a/CD18) and leads to subsequent cellular effects in a dose dependent manner. The objectives of this study were to delineate the mechanisms that underlie LktA-induced oncosis and apoptosis and to examine the role of LktA/LFA-1 interaction in these events. The results demonstrate that LktA-induced oncosis proceeds through a LFA-1 and caspase-1 dependent pathway referred to as 'pyrotosis', as well as through a LFA-1- and caspase-1-independent pathway. LktA-induced apoptosis in alveolar macrophages involves activation of caspase-3 and engages the extrinsic and intrinsic pathways of apoptosis, with the extrinsic pathway being dependent on LFA-1 signaling and TNFα.

AB - Mannheimia (Pasteurella) haemolytica leukotoxin (LktA) binds to the bovine β2 integrins (such as LFA-1 - CD11a/CD18) and leads to subsequent cellular effects in a dose dependent manner. The objectives of this study were to delineate the mechanisms that underlie LktA-induced oncosis and apoptosis and to examine the role of LktA/LFA-1 interaction in these events. The results demonstrate that LktA-induced oncosis proceeds through a LFA-1 and caspase-1 dependent pathway referred to as 'pyrotosis', as well as through a LFA-1- and caspase-1-independent pathway. LktA-induced apoptosis in alveolar macrophages involves activation of caspase-3 and engages the extrinsic and intrinsic pathways of apoptosis, with the extrinsic pathway being dependent on LFA-1 signaling and TNFα.

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KW - Caspase-8

KW - Mannheimia haemolytica leukotoxin

KW - Oncosis

KW - Pore formation

KW - Pyrotosis

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Mechanisms underlying Mannheimia haemolytica leukotoxin-induced oncosis and apoptosis of bovine alveolar macrophages

Abstract

Bibliographical note

Keywords

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