Mechanisms of occupational asthma: Not all allergens are equal

Jean F Regal, Amy L. Greene, Ronald R. Regal

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Asthma is a heterogeneous lung disorder characterized by airway obstruction, inflammation and eosinophil infiltration into the lung. Both genetics and environmental factors influence the expression of asthma, and not all asthma is the result of a specific immune response to allergen. Numerous asthma phenotypes have been described, including occupational asthma, and therapeutic strategies for asthma control are similar regardless of phenotype. We hypothesized that mechanistic pathways leading to asthma symptoms in the effector phase of the disorder differ with the inciting allergen. Since route of allergen exposure can influence mechanistic pathways, mice were sensitized by identical routes with a high molecular weight occupational allergen ovalbumin and a low molecular weight occupational allergen trimellitic anhydride (TMA). Different statistical methods with varying selection criteria resulted in identification of similar candidate genes. Array data are intended to provide candidate genes for hypothesis generation and further experimentation. Continued studies focused on genes showing minimal changes in the TMA-induced model but with clear up-regulation in the ovalbumin model. Two of these genes, arginase 1 and eotaxin 1 are the focus of continuing investigations in mouse models of asthma regarding differences in mechanistic pathways depending on the allergen. Microarray data from the ovalbumin and TMA model of asthma were also compared to previous data using Aspergillus as allergen to identify putative asthma 'signature genes', i.e. genes up-regulated with all 3 allergens. Array studies provide candidate genes to identify common mechanistic pathways in the effector phase, as well as mechanistic pathways unique to individual allergens.

Original languageEnglish (US)
Pages (from-to)165-171
Number of pages7
JournalEnvironmental Health and Preventive Medicine
Volume12
Issue number4
DOIs
StatePublished - Sep 10 2007

Fingerprint

Allergens
Occupational Asthma
Asthma
Genes
Ovalbumin
Chemokine CCL11
Molecular Weight
Molecular weight
Phenotype
Arginase
Lung
Aspergillus
Genetic Association Studies
Airway Obstruction
Microarrays
Infiltration
Eosinophils
Patient Selection
Statistical methods
Up-Regulation

Keywords

  • Arginase
  • Asthma
  • Eosinophils
  • Microarray
  • Trimellitic anhydride

Cite this

Mechanisms of occupational asthma : Not all allergens are equal. / Regal, Jean F; Greene, Amy L.; Regal, Ronald R.

In: Environmental Health and Preventive Medicine, Vol. 12, No. 4, 10.09.2007, p. 165-171.

Research output: Contribution to journalArticle

Regal, Jean F ; Greene, Amy L. ; Regal, Ronald R. / Mechanisms of occupational asthma : Not all allergens are equal. In: Environmental Health and Preventive Medicine. 2007 ; Vol. 12, No. 4. pp. 165-171.
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