Mechanical changes in the rat right ventricle with decellularization

Colleen Witzenburg, Ramesh Raghupathy, Stefan M. Kren, Doris A. Taylor, Victor H. Barocas

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

The stiffness, anisotropy, and heterogeneity of freshly dissected (control) and perfusion-decellularized rat right ventricles were compared using an anisotropic inverse mechanics method. Cruciform tissue samples were speckled and then tested under a series of different biaxial loading configurations with simultaneous force measurement on all four arms and displacement mapping via image correlation. Based on the displacement and force data, the sample was segmented into piecewise homogeneous partitions. Tissue stiffness and anisotropy were characterized for each partition using a large-deformation extension of the general linear elastic model. The perfusion-decellularized tissue had significantly higher stiffness than the control, suggesting that the cellular contribution to stiffness, at least under the conditions used, was relatively small. Neither anisotropy nor heterogeneity (measured by the partition standard deviation of the modulus and anisotropy) varied significantly between control and decellularized samples. We thus conclude that although decellularization produces quantitative differences in modulus, decellularized tissue can provide a useful model of the native tissue extracellular matrix. Further, the large-deformation inverse method presented herein can be used to characterize complex soft tissue behaviors.

Original languageEnglish (US)
Pages (from-to)842-849
Number of pages8
JournalJournal of Biomechanics
Volume45
Issue number5
DOIs
StatePublished - Mar 15 2012

Bibliographical note

Funding Information:
This work was supported in part by the National Institutes of Health (R21-EB-009788), the Medtronic Foundation, the National Heart Lung and Blood Institute’s Progenitor Cell Biology Consortium (#1U01HL100407-1), and the American Heart Association’s Jon Hold DeHaan Cardiac Myogenesis Research Center (#AHA09070499N). The technical assistance of Erich Boldt in preparing and testing samples is gratefully acknowledged. We also thank the Minnesota Supercomputing Institute for the computing resources.

Keywords

  • Biaxial testing
  • Heart ventricle
  • Heterogeneous
  • Perfusion-decellularization

Fingerprint

Dive into the research topics of 'Mechanical changes in the rat right ventricle with decellularization'. Together they form a unique fingerprint.

Cite this