Abstract
Online microdialysis-capillary electrophoresis (CE) was used to measure the changes in extracellular d-serine concentration in response to the application of glutamate agonists and antagonists in the rat striatum. The microdialysis-CE assay was capable of measuring concentration changes as small as 8% with a sampling rate of 12-15 s. Kainic acid (KA) induced increases in d- and l-serine concentrations. Application of the non-NMDA receptor antagonist CNQX did not affect the increases observed for d- or l-serine, suggesting a nonspecific effect. NMDA also induced increases in d-serine, l-serine, glutamate and GABA concentrations. These increases were attenuated by the NMDA receptor antagonist MK-801.
Original language | English (US) |
---|---|
Pages (from-to) | 200-205 |
Number of pages | 6 |
Journal | Neuroscience Letters |
Volume | 393 |
Issue number | 2-3 |
DOIs | |
State | Published - Jan 30 2006 |
Bibliographical note
Funding Information:The authors gratefully acknowledge funding provided by the National Institutes of Health (NS043304).
Keywords
- AMPA receptor
- Astrocyte
- Capillary electrophoresis
- Microdialysis
- NMDA receptor
- d-Serine