Measuring the effect of glutamate receptor agonists on extracellular d-serine concentrations in the rat striatum using online microdialysis-capillary electrophoresis

Chanda M. Ciriacks; Michael Bowser

doi:10.1016/j.neulet.2005.09.080

Measuring the effect of glutamate receptor agonists on extracellular d-serine concentrations in the rat striatum using online microdialysis-capillary electrophoresis

Chanda M. Ciriacks, Michael Bowser

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Online microdialysis-capillary electrophoresis (CE) was used to measure the changes in extracellular d-serine concentration in response to the application of glutamate agonists and antagonists in the rat striatum. The microdialysis-CE assay was capable of measuring concentration changes as small as 8% with a sampling rate of 12-15 s. Kainic acid (KA) induced increases in d- and l-serine concentrations. Application of the non-NMDA receptor antagonist CNQX did not affect the increases observed for d- or l-serine, suggesting a nonspecific effect. NMDA also induced increases in d-serine, l-serine, glutamate and GABA concentrations. These increases were attenuated by the NMDA receptor antagonist MK-801.

Original language	English (US)
Pages (from-to)	200-205
Number of pages	6
Journal	Neuroscience Letters
Volume	393
Issue number	2-3
DOIs	https://doi.org/10.1016/j.neulet.2005.09.080
State	Published - Jan 30 2006

Bibliographical note

Funding Information:
The authors gratefully acknowledge funding provided by the National Institutes of Health (NS043304).

Keywords

AMPA receptor
Astrocyte
Capillary electrophoresis
Microdialysis
NMDA receptor
d-Serine

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10.1016/j.neulet.2005.09.080

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title = "Measuring the effect of glutamate receptor agonists on extracellular d-serine concentrations in the rat striatum using online microdialysis-capillary electrophoresis",

abstract = "Online microdialysis-capillary electrophoresis (CE) was used to measure the changes in extracellular d-serine concentration in response to the application of glutamate agonists and antagonists in the rat striatum. The microdialysis-CE assay was capable of measuring concentration changes as small as 8\% with a sampling rate of 12-15 s. Kainic acid (KA) induced increases in d- and l-serine concentrations. Application of the non-NMDA receptor antagonist CNQX did not affect the increases observed for d- or l-serine, suggesting a nonspecific effect. NMDA also induced increases in d-serine, l-serine, glutamate and GABA concentrations. These increases were attenuated by the NMDA receptor antagonist MK-801.",

keywords = "AMPA receptor, Astrocyte, Capillary electrophoresis, Microdialysis, NMDA receptor, d-Serine",

author = "Ciriacks, \{Chanda M.\} and Michael Bowser",

note = "Funding Information: The authors gratefully acknowledge funding provided by the National Institutes of Health (NS043304).",

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doi = "10.1016/j.neulet.2005.09.080",

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AU - Ciriacks, Chanda M.

AU - Bowser, Michael

N1 - Funding Information: The authors gratefully acknowledge funding provided by the National Institutes of Health (NS043304).

PY - 2006/1/30

Y1 - 2006/1/30

N2 - Online microdialysis-capillary electrophoresis (CE) was used to measure the changes in extracellular d-serine concentration in response to the application of glutamate agonists and antagonists in the rat striatum. The microdialysis-CE assay was capable of measuring concentration changes as small as 8% with a sampling rate of 12-15 s. Kainic acid (KA) induced increases in d- and l-serine concentrations. Application of the non-NMDA receptor antagonist CNQX did not affect the increases observed for d- or l-serine, suggesting a nonspecific effect. NMDA also induced increases in d-serine, l-serine, glutamate and GABA concentrations. These increases were attenuated by the NMDA receptor antagonist MK-801.

AB - Online microdialysis-capillary electrophoresis (CE) was used to measure the changes in extracellular d-serine concentration in response to the application of glutamate agonists and antagonists in the rat striatum. The microdialysis-CE assay was capable of measuring concentration changes as small as 8% with a sampling rate of 12-15 s. Kainic acid (KA) induced increases in d- and l-serine concentrations. Application of the non-NMDA receptor antagonist CNQX did not affect the increases observed for d- or l-serine, suggesting a nonspecific effect. NMDA also induced increases in d-serine, l-serine, glutamate and GABA concentrations. These increases were attenuated by the NMDA receptor antagonist MK-801.

KW - AMPA receptor

KW - Astrocyte

KW - Capillary electrophoresis

KW - Microdialysis

KW - NMDA receptor

KW - d-Serine

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Measuring the effect of glutamate receptor agonists on extracellular d-serine concentrations in the rat striatum using online microdialysis-capillary electrophoresis

Abstract

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Keywords

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