Measuring the effect of crystalline order on DNA electrophoresis in colloidal crystals

Scott B. King, Kevin D. Dorfman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Colloidal crystals can be used as sieves for DNA electrophoresis in microfabricated devices. A key difference between crystals and agarose gels is the ordered nature of colloidal crystals. Unlike top-down microfabricated DNA separation devices, colloidal crystals are not perfectly ordered. In most self-assembled crystals, there exists a number of crystalline grains of varying size and orientation. We use a combination of laser diffraction to qualify the crystalline order and high-density measurements of DNA electrophoresis to compare separation performance in regions of high and low crystallinity. We find that for small DNA crystalline order plays no role in DNA separation performance [1].

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1860-1862
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • Colloidal crystals
  • DNA electrophoresis
  • Laser diffraction
  • Ogston sieving

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