Measuring pressure volume loops in the mouse

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Understanding the causes and progression of heart disease presents a significant challenge to the biomedical community. The genetic flexibility of the mouse provides great potential to explore cardiac function at the molecular level. The mouse’s small size does present some challenges in regards to performing detailed cardiac phenotyping. Miniaturization and other advancements in technology have made many methods of cardiac assessment possible in the mouse. Of these, the simultaneous collection of pressure and volume data provides a detailed picture of cardiac function that is not available through any other modality. Here a detailed procedure for the collection of pressure-volume loop data is described. Included is a discussion of the principles underlying the measurements and the potential sources of error. Anesthetic management and surgical approaches are discussed in great detail as they are both critical to obtaining high quality hemodynamic measurements. The principles of hemodynamic protocol development and relevant aspects of data analysis are also addressed.

Original languageEnglish (US)
Article numbere53810
JournalJournal of Visualized Experiments
Volume2016
Issue number111
DOIs
StatePublished - May 2 2016

Bibliographical note

Funding Information:
The author would like to acknowledge funding from NHLBI (K08 HL102066 and R01 HL114832).

Publisher Copyright:
© 2016 Journal of Visualized Experiments.

Keywords

  • Cardiac physiology
  • Contractility
  • In vivo Hemodynamics
  • Issue 111
  • Mouse
  • Physiology
  • Pressure-volume loops
  • Ventricular loading

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