Here we describe an in vivo dye-tracking method for measuring phloem transport velocity in seedlings, leaves and petioles and potentially other translucent plant tissues. The method requires measurement of the fluorescent signal of a phloem-mobile fluorescent dye using sensitive photo-sensors placed external to the plant. Following dye application, velocity is determined using laser fluorescence bleaching and measuring the time it takes for the bleach front to reach a light sensor. This method can be used to measure phloem transport velocity on intact plants with minimal disturbance and has a potential to be used under a variety of growth conditions and in the field. Because there are large differences among species in their anatomy, this method should be optimized to individual plants and tissue types.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||9|
|State||Published - 2019|
|Name||Methods in molecular biology (Clifton, N.J.)|
Bibliographical noteFunding Information:
This work was partially supported by the National Science Foundation Integrative Organismal Systems 1656318 (JAS).
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.
- Carbon allocation
- Carboxyfluorescein diacetate
- Fluorescence bleaching
- Phloem-mobile dyes
- Transport velocity
PubMed: MeSH publication types
- Research Support, Non-U.S. Gov't
- Journal Article