Measuring Phloem Transport Velocity on a Tissue Level Using a Phloem-Mobile Dye

Jessica A. Savage, Maciej A. Zwieniecki

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Here we describe an in vivo dye-tracking method for measuring phloem transport velocity in seedlings, leaves and petioles and potentially other translucent plant tissues. The method requires measurement of the fluorescent signal of a phloem-mobile fluorescent dye using sensitive photo-sensors placed external to the plant. Following dye application, velocity is determined using laser fluorescence bleaching and measuring the time it takes for the bleach front to reach a light sensor. This method can be used to measure phloem transport velocity on intact plants with minimal disturbance and has a potential to be used under a variety of growth conditions and in the field. Because there are large differences among species in their anatomy, this method should be optimized to individual plants and tissue types.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages203-211
Number of pages9
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2014
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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Keywords

  • Carbon allocation
  • Carboxyfluorescein diacetate
  • FRAP
  • Fluorescence
  • Fluorescence bleaching
  • Phloem-mobile dyes
  • Transport velocity

Cite this

Savage, J. A., & Zwieniecki, M. A. (2019). Measuring Phloem Transport Velocity on a Tissue Level Using a Phloem-Mobile Dye. In Methods in Molecular Biology (pp. 203-211). (Methods in Molecular Biology; Vol. 2014). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9562-2_17