Measuring percent lymphocytes by flow cytometry to calculate absolute lymphocyte subset counts for HIV + specimens

Howard B. Gale, Keith Henry

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

A comparison was made of lymphocyte percentages from an automated hematology analyzer (ELT 15) vs. a fluorescence flow cytometer (Cytofluorograf). The hematology values were consistently higher than the flow (by greater than 10% for 13 of 50 HIV + specimens). The findings were similar to three other pairings: H*1 vs. Cytofluorograf, Cell Dyn 3000 vs. Cytofluorograf, and Cell Dyn 3000 vs. EPICS Profile. In another comparison manual percent lymphocytes matched much better with flow values. Factors of sample preparation and instrument analysis as they relate to nonrandom cell loss and lymphocyte resolution are examined.

Original languageEnglish (US)
Pages (from-to)175-181
Number of pages7
JournalCytometry
Volume13
Issue number2
DOIs
StatePublished - 1992

Keywords

  • AIDS
  • CD4 count
  • light scattering
  • method
  • quality control

Fingerprint Dive into the research topics of 'Measuring percent lymphocytes by flow cytometry to calculate absolute lymphocyte subset counts for HIV + specimens'. Together they form a unique fingerprint.

Cite this