Measuring biological age in mice using differential mass spectrometry

Harris Bell-Temin, Matthew J. Yousefzadeh, Andrey Bondarenko, Ellen Quarles, Jacqueline Jones-Laughner, Paul D. Robbins, Warren Ladiges, Laura J. Niedernhofer, Nathan A. Yates

Research output: Contribution to journalArticle

Abstract

Aging is an ill-defined process that increases the risk of morbidity and mortality. Aging is also heterogeneous meaning that biological and chronological age can differ. Here, we used unbiased differential mass spectrometry to quantify thousands of proteins in mouse liver and select those that that consistently change in expression as mice age. A panel of 14 proteins from inbred C57BL/6 mice was used to equate chronological and biological age in this reference population, against which other mice could be compared. This "biological age calculator" identified two strains of f1 hybrid mice as biologically younger than inbred mice and progeroid mice as being biologically older. In an independent validation experiment, the calculator identified mice treated with rapamycin, known to extend lifespan of mice, as 18% younger than mice fed a placebo diet. This demonstrates that it is possible to measure subtle changes in biologic age in mammals using a proteomics approach.

Original languageEnglish (US)
Pages (from-to)1045-1061
Number of pages17
JournalAging
Volume11
Issue number3
DOIs
StatePublished - Jan 1 2019

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Mass Spectrometry
Sirolimus
Inbred C57BL Mouse
Proteomics
Mammals
Proteins
Placebos
Diet
Morbidity
Mortality
Liver
Population

Keywords

  • Aging
  • Liver
  • Mass spectrometry
  • Mouse
  • Non-targeted
  • Proteomics
  • Surrogate markers

Cite this

Bell-Temin, H., Yousefzadeh, M. J., Bondarenko, A., Quarles, E., Jones-Laughner, J., Robbins, P. D., ... Yates, N. A. (2019). Measuring biological age in mice using differential mass spectrometry. Aging, 11(3), 1045-1061. https://doi.org/10.18632/aging.101810

Measuring biological age in mice using differential mass spectrometry. / Bell-Temin, Harris; Yousefzadeh, Matthew J.; Bondarenko, Andrey; Quarles, Ellen; Jones-Laughner, Jacqueline; Robbins, Paul D.; Ladiges, Warren; Niedernhofer, Laura J.; Yates, Nathan A.

In: Aging, Vol. 11, No. 3, 01.01.2019, p. 1045-1061.

Research output: Contribution to journalArticle

Bell-Temin, H, Yousefzadeh, MJ, Bondarenko, A, Quarles, E, Jones-Laughner, J, Robbins, PD, Ladiges, W, Niedernhofer, LJ & Yates, NA 2019, 'Measuring biological age in mice using differential mass spectrometry', Aging, vol. 11, no. 3, pp. 1045-1061. https://doi.org/10.18632/aging.101810
Bell-Temin H, Yousefzadeh MJ, Bondarenko A, Quarles E, Jones-Laughner J, Robbins PD et al. Measuring biological age in mice using differential mass spectrometry. Aging. 2019 Jan 1;11(3):1045-1061. https://doi.org/10.18632/aging.101810
Bell-Temin, Harris ; Yousefzadeh, Matthew J. ; Bondarenko, Andrey ; Quarles, Ellen ; Jones-Laughner, Jacqueline ; Robbins, Paul D. ; Ladiges, Warren ; Niedernhofer, Laura J. ; Yates, Nathan A. / Measuring biological age in mice using differential mass spectrometry. In: Aging. 2019 ; Vol. 11, No. 3. pp. 1045-1061.
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