Abstract
We present a method for measuring the difference in stretch between GC and AT rich regions within genomic DNA from a human cell line NA 12878. Individual molecules of long DNA contain fluorescent labels at a 7 base recognition site from Nt.BspQI and are confined in an array of nanochannels allowing for the molecules to be aligned to the human reference. Data was collected on over 450,000 DNA molecules and once aligned, the fluorescent nick sites within individual molecules provided a precise ruler for measuring stretch based on the known GC content within a pair of nick sites.
| Original language | English (US) |
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| Title of host publication | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 289-290 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780692941836 |
| State | Published - Jan 1 2020 |
| Event | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States Duration: Oct 22 2017 → Oct 26 2017 |
Publication series
| Name | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 |
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Conference
| Conference | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 |
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| Country/Territory | United States |
| City | Savannah |
| Period | 10/22/17 → 10/26/17 |
Bibliographical note
Funding Information:This work was funded by the NIH, grant R01-HG006851.
Publisher Copyright:
© 17CBMS-0001.
Keywords
- DNA
- GC content
- Nanochannel
- Stretch