Measurement of tissue oxygen saturation levels using portable near-infrared spectroscopy in clinically healthy dogs

Kelly E. Hall, Lisa L. Powell, Gregory J Beilman, Karin R. Shafer, Vickie K. Skala, Elizabeth A. Olmstead

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Objective: To establish a reference interval for tissue oxygen saturation (StO2) levels measured by a portable near-infrared spectroscope and determine site(s) for reproducibly measuring StO2 levels in dogs. Design Prospective experimental study. Setting Veterinary teaching hospital. Animals Seventy-eight healthy dogs. Measurements and Main Results: A portable device that quantitatively measures StO2 levels directly in muscle tissue using near-infrared spectroscopy (NIRS) was topically applied to shaved sites over 4 muscle bodies. Readings from the sartorius muscle were obtained 100% of the time. The digital extensors and biceps femoris muscles provided similar readings, but less consistently obtained StO2 values (70% and 67%, respectively). Mean StO2 levels measured over these 3 sites were not statistically different from one another. When readings from these 3 sites were combined, a mean ±1 SD of 92.9±7.4% was obtained. The epaxial muscles produced a significantly lower mean ±1 SD (68.5±22.4%), and readings were obtained only 60% of the time. Conclusions In dogs, a mean ±1 SD of 92.9±7.4% can be used to investigate clinical applications of NIRS. The sartorius muscle most consistently allows for detection of StO2 levels (100%). The epaxial muscles are not consistent or reliable for obtaining StO2 readings and are not recommended for clinical application of near-infrared spectroscope. Sex does not significantly affect StO2 readings at any site. Body condition score only affects readings obtained from the sartorius muscle.

Original languageEnglish (US)
Pages (from-to)594-600
Number of pages7
JournalJournal of Veterinary Emergency and Critical Care
Issue number6
StatePublished - Dec 2008


  • Patient monitoring
  • Perfusion
  • Reference values


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