Measurement of the distribution of aerosols among mouse lobes by fluorescent imaging

Dandan Yi, Andrew Price, Angela Panoskaltsis-Mortari, Amir Naqwi, Timothy Scott Wiedmann

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Lung samples were prepared to investigate the perturbing effects of light absorption for quantifying the fluorescence signal of aluminum phthalocyanine tetrasulfonic acid (AlPCS). Standard solutions of known concentration and depth were imaged with different exposure times and analyzed. The intensity was found to be a linear function of concentration, depth, exposure time, and area. Mice were exposed to an aerosol of AlPCS with a mass median aerodynamic diameter of 390. nm and geometric standard deviation of 1.8. Images of intact lung lobes and lung homogenates were obtained and then analyzed to allow quantifying the concentration of AlPCS among the lung lobes and trachea. For the distribution of aerosols, the results indicate that the concentration was uniform among the different lobes. Combining the quantitative analysis of the concentration with image analysis of the area/thickness, the mass deposited in each lobe was readily determined. This approach provides a quantitative means to determine the selectivity of drug delivery to mouse lower respiratory tract.

Original languageEnglish (US)
Pages (from-to)88-93
Number of pages6
JournalAnalytical Biochemistry
Volume403
Issue number1-2
DOIs
StatePublished - Aug 2010

Keywords

  • Aerosol
  • Fluorescent imaging
  • Lung lobes

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