TY - JOUR
T1 - Measurement of the distribution of aerosols among mouse lobes by fluorescent imaging
AU - Yi, Dandan
AU - Price, Andrew
AU - Panoskaltsis-Mortari, Angela
AU - Naqwi, Amir
AU - Wiedmann, Timothy Scott
N1 - Funding Information:
Financial support was provided in part by NIH Grant HL081789 (A.N.). D.Y. was supported by a fellowship from China Scholarship Council.
PY - 2010/8
Y1 - 2010/8
N2 - Lung samples were prepared to investigate the perturbing effects of light absorption for quantifying the fluorescence signal of aluminum phthalocyanine tetrasulfonic acid (AlPCS). Standard solutions of known concentration and depth were imaged with different exposure times and analyzed. The intensity was found to be a linear function of concentration, depth, exposure time, and area. Mice were exposed to an aerosol of AlPCS with a mass median aerodynamic diameter of 390. nm and geometric standard deviation of 1.8. Images of intact lung lobes and lung homogenates were obtained and then analyzed to allow quantifying the concentration of AlPCS among the lung lobes and trachea. For the distribution of aerosols, the results indicate that the concentration was uniform among the different lobes. Combining the quantitative analysis of the concentration with image analysis of the area/thickness, the mass deposited in each lobe was readily determined. This approach provides a quantitative means to determine the selectivity of drug delivery to mouse lower respiratory tract.
AB - Lung samples were prepared to investigate the perturbing effects of light absorption for quantifying the fluorescence signal of aluminum phthalocyanine tetrasulfonic acid (AlPCS). Standard solutions of known concentration and depth were imaged with different exposure times and analyzed. The intensity was found to be a linear function of concentration, depth, exposure time, and area. Mice were exposed to an aerosol of AlPCS with a mass median aerodynamic diameter of 390. nm and geometric standard deviation of 1.8. Images of intact lung lobes and lung homogenates were obtained and then analyzed to allow quantifying the concentration of AlPCS among the lung lobes and trachea. For the distribution of aerosols, the results indicate that the concentration was uniform among the different lobes. Combining the quantitative analysis of the concentration with image analysis of the area/thickness, the mass deposited in each lobe was readily determined. This approach provides a quantitative means to determine the selectivity of drug delivery to mouse lower respiratory tract.
KW - Aerosol
KW - Fluorescent imaging
KW - Lung lobes
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U2 - 10.1016/j.ab.2010.04.005
DO - 10.1016/j.ab.2010.04.005
M3 - Article
C2 - 20382107
AN - SCOPUS:77953358101
VL - 403
SP - 88
EP - 93
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1-2
ER -