Measurement of the caspase-1-like activity of vacuolar processing enzyme in plants

Noriyuki Hatsugai, Ikuko Hara-Nishimura

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Caspase-like activities are essential to regulate programed cell death in plants. Although no caspase orthologous enzymes with aspartic acid specificity have been identified in plants, vacuolar processing enzyme (VPE) exhibits a caspase-1-like activity. In this chapter, we introduce two methods for the measurement of the caspase-1-like/VPE activity. These methods are based on the cleavage of caspase-1 specific synthetic substrates and on monitoring the active forms of VPE using a biotinylated-inhibitor blot analysis. Both methods are also adaptable to other plant caspase-like activities.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This work was supported by Grants-in-Aid for Scientific Research (no. 15H05776) to I.H-.N. from Japan Society for the Promotion of Science (JSPS).

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC.


  • Caspase-1-like activity
  • Plants
  • VPE
  • Vacuolar processing enzyme


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