Measurement of the caspase-1-like activity of vacuolar processing enzyme in plants

Noriyuki Hatsugai, Ikuko Hara-Nishimura

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Caspase-like activities are essential to regulate programed cell death in plants. Although no caspase orthologous enzymes with aspartic acid specificity have been identified in plants, vacuolar processing enzyme (VPE) exhibits a caspase-1-like activity. In this chapter, we introduce two methods for the measurement of the caspase-1-like/VPE activity. These methods are based on the cleavage of caspase-1 specific synthetic substrates and on monitoring the active forms of VPE using a biotinylated-inhibitor blot analysis. Both methods are also adaptable to other plant caspase-like activities.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages163-171
Number of pages9
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1743
ISSN (Print)1064-3745

Keywords

  • Caspase-1-like activity
  • Plants
  • VPE
  • Vacuolar processing enzyme

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