Measurement of Resting and Stress‐elevated Serum Cortisol in Rainbow Trout Oncorhynchus mykiss in Experimental Net‐pens

Myron J. Kebus; Michael T. Collins; Mark S. Brownfield; Clyde H. Amundson; Terrence B. Kayes; Jeffrey A. Malison

doi:10.1111/j.1749-7345.1992.tb00754.x

Measurement of Resting and Stress‐elevated Serum Cortisol in Rainbow Trout Oncorhynchus mykiss in Experimental Net‐pens

Myron J. Kebus, Michael T. Collins, Mark S. Brownfield, Clyde H. Amundson, Terrence B. Kayes, Jeffrey A. Malison

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A commercially available heterogeneous, solid‐phase tube enzyme‐linked immunoassay (ELISA) was modified and validated for the measurement of serum cortisol in rainbow trout Oncorhynchus mykiss. The assay is accurate and precise. Resting and stress‐elevated serum cortisol concentrations were measured in rainbow trout with a sensitivity of 1.5 ng/ml. Fish held in net‐pens at a density of 0.4 kg/m³/cm had a resting cortisol level of 16.5 ± 3.8 ng/ml (mean ± SE). At 3 h postdisturbance, serum cortisol levels were not affected by the removal of fish from adjacent net‐pens with dip nets or by the use of 200 mg/L tricaine methanesulfonate (MS‐222) as an anesthetic for obtaining samples. However, an acute stress (60 s removal from water) elevated serum cortisol levels to 73.7 ± 9.4 ng/ml.

Original language	English (US)
Pages (from-to)	83-88
Number of pages	6
Journal	Journal of the World Aquaculture Society
Volume	23
Issue number	1
DOIs	https://doi.org/10.1111/j.1749-7345.1992.tb00754.x
State	Published - Mar 1992
Externally published	Yes

Publisher link

10.1111/j.1749-7345.1992.tb00754.x

Find It

Find It at U of M Libraries

Cite this

@article{cde766c24f044f84a34ff29c9564db4a,

title = "Measurement of Resting and Stress‐elevated Serum Cortisol in Rainbow Trout Oncorhynchus mykiss in Experimental Net‐pens",

abstract = "A commercially available heterogeneous, solid‐phase tube enzyme‐linked immunoassay (ELISA) was modified and validated for the measurement of serum cortisol in rainbow trout Oncorhynchus mykiss. The assay is accurate and precise. Resting and stress‐elevated serum cortisol concentrations were measured in rainbow trout with a sensitivity of 1.5 ng/ml. Fish held in net‐pens at a density of 0.4 kg/m3/cm had a resting cortisol level of 16.5 ± 3.8 ng/ml (mean ± SE). At 3 h postdisturbance, serum cortisol levels were not affected by the removal of fish from adjacent net‐pens with dip nets or by the use of 200 mg/L tricaine methanesulfonate (MS‐222) as an anesthetic for obtaining samples. However, an acute stress (60 s removal from water) elevated serum cortisol levels to 73.7 ± 9.4 ng/ml.",

author = "Kebus, {Myron J.} and Collins, {Michael T.} and Brownfield, {Mark S.} and Amundson, {Clyde H.} and Kayes, {Terrence B.} and Malison, {Jeffrey A.}",

year = "1992",

month = mar,

doi = "10.1111/j.1749-7345.1992.tb00754.x",

language = "English (US)",

volume = "23",

pages = "83--88",

journal = "Journal of the World Aquaculture Society",

issn = "0893-8849",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Measurement of Resting and Stress‐elevated Serum Cortisol in Rainbow Trout Oncorhynchus mykiss in Experimental Net‐pens

AU - Kebus, Myron J.

AU - Collins, Michael T.

AU - Brownfield, Mark S.

AU - Amundson, Clyde H.

AU - Kayes, Terrence B.

AU - Malison, Jeffrey A.

PY - 1992/3

Y1 - 1992/3

N2 - A commercially available heterogeneous, solid‐phase tube enzyme‐linked immunoassay (ELISA) was modified and validated for the measurement of serum cortisol in rainbow trout Oncorhynchus mykiss. The assay is accurate and precise. Resting and stress‐elevated serum cortisol concentrations were measured in rainbow trout with a sensitivity of 1.5 ng/ml. Fish held in net‐pens at a density of 0.4 kg/m3/cm had a resting cortisol level of 16.5 ± 3.8 ng/ml (mean ± SE). At 3 h postdisturbance, serum cortisol levels were not affected by the removal of fish from adjacent net‐pens with dip nets or by the use of 200 mg/L tricaine methanesulfonate (MS‐222) as an anesthetic for obtaining samples. However, an acute stress (60 s removal from water) elevated serum cortisol levels to 73.7 ± 9.4 ng/ml.

AB - A commercially available heterogeneous, solid‐phase tube enzyme‐linked immunoassay (ELISA) was modified and validated for the measurement of serum cortisol in rainbow trout Oncorhynchus mykiss. The assay is accurate and precise. Resting and stress‐elevated serum cortisol concentrations were measured in rainbow trout with a sensitivity of 1.5 ng/ml. Fish held in net‐pens at a density of 0.4 kg/m3/cm had a resting cortisol level of 16.5 ± 3.8 ng/ml (mean ± SE). At 3 h postdisturbance, serum cortisol levels were not affected by the removal of fish from adjacent net‐pens with dip nets or by the use of 200 mg/L tricaine methanesulfonate (MS‐222) as an anesthetic for obtaining samples. However, an acute stress (60 s removal from water) elevated serum cortisol levels to 73.7 ± 9.4 ng/ml.

UR - http://www.scopus.com/inward/record.url?scp=33645400745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645400745&partnerID=8YFLogxK

U2 - 10.1111/j.1749-7345.1992.tb00754.x

DO - 10.1111/j.1749-7345.1992.tb00754.x

M3 - Article

AN - SCOPUS:33645400745

SN - 0893-8849

VL - 23

SP - 83

EP - 88

JO - Journal of the World Aquaculture Society

JF - Journal of the World Aquaculture Society

IS - 1

ER -

Measurement of Resting and Stress‐elevated Serum Cortisol in Rainbow Trout Oncorhynchus mykiss in Experimental Net‐pens

Abstract

Publisher link

Find It

Other files and links

Fingerprint

Cite this