Measurement of low-density-lipoprotein cholesterol in serum: A status report

N. Rifai, G. R. Warnick, J. R. McNamara, J. D. Belcher, G. F. Grinstead, I. D. Frantz

Research output: Contribution to journalArticle

135 Scopus citations


Current recommendations of the Adult Treatment Panel and the Children and Adolescents Treatment Panel of the National Cholesterol Education Program make the concentration of low-density lipoproteins cholesterol (LDL-C) in serum the basis for the classification and treatment of hypercholesterolemia. Numerous methodologies for the determination of serum LDL-C concentrations, in research and clinical laboratories, have been described. Here, we review the principles, performance, and limitations of major current methodologies for determining LDL-C concentrations. These methods include sequential and density-gradient ultracentrifugation, chromatographic and electrophoretic techniques, and precipitation methods. In addition, the advantages and disadvantages of estimating LDL-C concentration by the Friedewald equation, the most commonly used approach in clinical laboratories, are addressed.

Original languageEnglish (US)
Pages (from-to)150-160
Number of pages11
JournalClinical chemistry
Issue number1
StatePublished - 1992


  • apolipoproteins
  • heart disease
  • risk factor

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