We describe a simple technique to measure sulfide in fecal homogenates (or any other liquid milieu), which involves acidification followed by the G.C. measurement of H2S in a gas space equilibrated with a small quantity of homogenate. An internal standard of Zn35S added to the homogenate permits correction for incomplete recovery of H2S in the gas space. The use of a sulfur chemiluminescence detector, which specifically and sensitively responds to sulfur-containing compounds, greatly facilitates this measurement.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|State||Published - Apr 15 2001|
Bibliographical noteFunding Information:
Supported in part by General Medical Research funds from the U.S. Department of Veterans Affairs and The National Institute of Diabetes and Digestive and Kidney Diseases grant R01 DK 13309-25.