Measurement of fecal sulfide using gas chromatography and a sulfur chemiluminescence detector

Julie K. Furne, John Springfield, Thomas Koenig, Fabrizis Suarez, Michael D. Levitt

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We describe a simple technique to measure sulfide in fecal homogenates (or any other liquid milieu), which involves acidification followed by the G.C. measurement of H2S in a gas space equilibrated with a small quantity of homogenate. An internal standard of Zn35S added to the homogenate permits correction for incomplete recovery of H2S in the gas space. The use of a sulfur chemiluminescence detector, which specifically and sensitively responds to sulfur-containing compounds, greatly facilitates this measurement.

Original languageEnglish (US)
Pages (from-to)253-258
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume754
Issue number1
DOIs
StatePublished - Apr 15 2001

Keywords

  • Sulfide

Fingerprint Dive into the research topics of 'Measurement of fecal sulfide using gas chromatography and a sulfur chemiluminescence detector'. Together they form a unique fingerprint.

Cite this