Abstract
We present a theoretical model and experimental data on the entropic unfolding force within DNA confined in nanochannels. We implemented a high throughput device that contains an array of 12,860 nanochannels with a 45nm cross-section capable of loading long DNA molecules (> 50 kbp) through electrophoresis. Upon insertion into the nanochannels, approximately half of the DNA molecules contain a short-lived, small fold on the leading edge that unfolds through entropic forces. Understanding the polymer physics of this system has implications for genomic mapping, as the small folds within DNA could appear as false mutation events when compared to the reference.
| Original language | English (US) |
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| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1089-1090 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| State | Published - 2016 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: Oct 9 2016 → Oct 13 2016 |
Publication series
| Name | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
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Other
| Other | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
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| Country/Territory | Ireland |
| City | Dublin |
| Period | 10/9/16 → 10/13/16 |
Bibliographical note
Funding Information:This work was funded by the NIH, grant R01-HG006851.
Keywords
- DNA
- Folds
- Nanochannel
- Odijk regime