Measurement of deubiquitinating enzyme activity via a suicidal HA-Ub-VS probe

Colleen Rivard; Martina Bazzaro

doi:10.1007/978-1-4939-2013-6_14

Measurement of deubiquitinating enzyme activity via a suicidal HA-Ub-VS probe

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Deubiquitinating enzymes (DUBs) are a novel "drug-able" target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.

Original language	English (US)
Title of host publication	Cervical Cancer
Subtitle of host publication	Methods and Protocols
Publisher	Springer New York
Pages	193-200
Number of pages	8
ISBN (Electronic)	9781493920136
ISBN (Print)	9781493920129
DOIs	https://doi.org/10.1007/978-1-4939-2013-6_14
State	Published - Oct 27 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015. All rights are reserved.

Keywords

Cervical cancer
Cysteine proteases
Deubiquitinating enzymes
Dub
Ha-ub-vs probe
Metalloproteases
P53
Protease inhibitors
Proteases
Proteasome
Ubiquitin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-2013-6_14

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author = "Colleen Rivard and Martina Bazzaro",

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doi = "10.1007/978-1-4939-2013-6_14",

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AU - Rivard, Colleen

AU - Bazzaro, Martina

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015. All rights are reserved.

PY - 2014/10/27

Y1 - 2014/10/27

N2 - Deubiquitinating enzymes (DUBs) are a novel "drug-able" target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.

AB - Deubiquitinating enzymes (DUBs) are a novel "drug-able" target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.

KW - Cervical cancer

KW - Cysteine proteases

KW - Deubiquitinating enzymes

KW - Dub

KW - Ha-ub-vs probe

KW - Metalloproteases

KW - P53

KW - Protease inhibitors

KW - Proteases

KW - Proteasome

KW - Ubiquitin

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DO - 10.1007/978-1-4939-2013-6_14

M3 - Chapter

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AN - SCOPUS:84958662477

SN - 9781493920129

SP - 193

EP - 200

BT - Cervical Cancer

PB - Springer New York

ER -

Measurement of deubiquitinating enzyme activity via a suicidal HA-Ub-VS probe

Abstract

Bibliographical note

Keywords

UN SDGs

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