Measurement of deubiquitinating enzyme activity via a suicidal HA-UB-VS probe

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9 Scopus citations


Deubiquitinating enzymes (DUBs) are a novel “drug-able” target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.

Original languageEnglish (US)
Pages (from-to)193-200
Number of pages8
JournalMethods in Molecular Biology
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.


  • Cervical cancer
  • Cysteine proteases
  • DUB
  • Deubiquitinating enzymes
  • HA-Ub-VS probe
  • Metalloproteases
  • P53
  • Protease inhibitors
  • Proteases
  • Proteasome
  • Ubiquitin


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