Deubiquitinating enzymes (DUBs) are a novel “drug-able” target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.
|Original language||English (US)|
|Number of pages||8|
|Journal||Methods in Molecular Biology|
|State||Published - 2015|
Bibliographical notePublisher Copyright:
© Springer Science+Business Media New York 2015.
- Cervical cancer
- Cysteine proteases
- Deubiquitinating enzymes
- HA-Ub-VS probe
- Protease inhibitors