Abstract
Deubiquitinating enzymes (DUBs) are a novel “drug-able” target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.
Original language | English (US) |
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Pages (from-to) | 193-200 |
Number of pages | 8 |
Journal | Methods in Molecular Biology |
Volume | 1249 |
DOIs | |
State | Published - 2015 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media New York 2015.
Keywords
- Cervical cancer
- Cysteine proteases
- DUB
- Deubiquitinating enzymes
- HA-Ub-VS probe
- Metalloproteases
- P53
- Protease inhibitors
- Proteases
- Proteasome
- Ubiquitin