Measurement of cyclic nucleotides in histologically defined samples of brain and retina

J. A. Ferrendelli, E. H. Rubin, H. T. Orr, D. A. Kinscherf, O. H. Lowry

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A method is described which allows measurement of cyclic AMP and cyclic GMP in histologically defined tissue samples containing as little as 0.6 fmol of either compound. This permits, for example, assays of freeze-dried samples as small as 0.03 μg dry weight from individual retina layers or 4-μg samples from individual layers of cerebellum. The procedure involves a modified radioimmunoassay at reduced volume for acetylated cyclic nucleotides in combination with some of the techniques previously developed for quantitative histochemical assays.

Original languageEnglish (US)
Pages (from-to)252-259
Number of pages8
JournalAnalytical Biochemistry
Volume78
Issue number1
DOIs
StatePublished - Mar 1977

Fingerprint

Cyclic Nucleotides
Retina
Assays
Brain
Cyclic GMP
Cyclic AMP
Cerebellum
Radioimmunoassay
Tissue
Weights and Measures

Cite this

Measurement of cyclic nucleotides in histologically defined samples of brain and retina. / Ferrendelli, J. A.; Rubin, E. H.; Orr, H. T.; Kinscherf, D. A.; Lowry, O. H.

In: Analytical Biochemistry, Vol. 78, No. 1, 03.1977, p. 252-259.

Research output: Contribution to journalArticle

Ferrendelli, J. A. ; Rubin, E. H. ; Orr, H. T. ; Kinscherf, D. A. ; Lowry, O. H. / Measurement of cyclic nucleotides in histologically defined samples of brain and retina. In: Analytical Biochemistry. 1977 ; Vol. 78, No. 1. pp. 252-259.
@article{89ad6ff216964c8ca8ff9cc2c82a1237,
title = "Measurement of cyclic nucleotides in histologically defined samples of brain and retina",
abstract = "A method is described which allows measurement of cyclic AMP and cyclic GMP in histologically defined tissue samples containing as little as 0.6 fmol of either compound. This permits, for example, assays of freeze-dried samples as small as 0.03 μg dry weight from individual retina layers or 4-μg samples from individual layers of cerebellum. The procedure involves a modified radioimmunoassay at reduced volume for acetylated cyclic nucleotides in combination with some of the techniques previously developed for quantitative histochemical assays.",
author = "Ferrendelli, {J. A.} and Rubin, {E. H.} and Orr, {H. T.} and Kinscherf, {D. A.} and Lowry, {O. H.}",
year = "1977",
month = "3",
doi = "10.1016/0003-2697(77)90030-6",
language = "English (US)",
volume = "78",
pages = "252--259",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Measurement of cyclic nucleotides in histologically defined samples of brain and retina

AU - Ferrendelli, J. A.

AU - Rubin, E. H.

AU - Orr, H. T.

AU - Kinscherf, D. A.

AU - Lowry, O. H.

PY - 1977/3

Y1 - 1977/3

N2 - A method is described which allows measurement of cyclic AMP and cyclic GMP in histologically defined tissue samples containing as little as 0.6 fmol of either compound. This permits, for example, assays of freeze-dried samples as small as 0.03 μg dry weight from individual retina layers or 4-μg samples from individual layers of cerebellum. The procedure involves a modified radioimmunoassay at reduced volume for acetylated cyclic nucleotides in combination with some of the techniques previously developed for quantitative histochemical assays.

AB - A method is described which allows measurement of cyclic AMP and cyclic GMP in histologically defined tissue samples containing as little as 0.6 fmol of either compound. This permits, for example, assays of freeze-dried samples as small as 0.03 μg dry weight from individual retina layers or 4-μg samples from individual layers of cerebellum. The procedure involves a modified radioimmunoassay at reduced volume for acetylated cyclic nucleotides in combination with some of the techniques previously developed for quantitative histochemical assays.

UR - http://www.scopus.com/inward/record.url?scp=0017349289&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017349289&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(77)90030-6

DO - 10.1016/0003-2697(77)90030-6

M3 - Article

C2 - 192102

AN - SCOPUS:0017349289

VL - 78

SP - 252

EP - 259

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -