Measurement of changes in urinary neopterin and total neopterin in body builders using SCX HPLC

Angus Lindsay, Tejraj Janmale, Nick Draper, Steven P. Gieseg

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Body building is a sport where ultrastructual damage to muscle fibres aids the development of dense muscle layers. Using a new strong cation exchange (SCX) based chromatography technique to measure neopterin and 7,8-dihydroneopterin, we investigated whether this muscle damage caused increased levels of inflammation. Urine samples were collected over eight consecutive mornings from 10 natural competitive body builders. Samples were analysed using SCX high performance liquid chromatography (HPLC) with urine volume corrected for creatinine and specific gravity (SG). The majority of subjects showed large changes in both neopterin and total neopterin (7,8-dihydroneopterin+neopterin) levels, though the mean data for the group showed no significant change over the week. There was no evidence of the high intensity resistance training causing an accumulation of inflammation as the values for all the body builders returned to close to the starting values after 2 days rest. The SCX analysis had an intra-specific viability of 3.04% and the inter-specific viability was 5.42%. Urine volume correction with SG was found to give the same values as using creatinine. Creatinine and specific gravity are both reliable methods for correcting for urine volume while SCX HPLC provides a new means of measuring urinary neopterin and total neopterin.

Original languageEnglish (US)
Pages (from-to)53-62
Number of pages10
Issue number2
StatePublished - Apr 1 2014

Bibliographical note

Funding Information:
Acknowledgments: This work was supported by the Free Radical Biochemistry Laboratory in the School of Biological Sciences, University of Canterbury.


  • Body building
  • Chromatography
  • Creatinine
  • Neopterin
  • Specific gravity
  • Urine

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