TY - JOUR
T1 - Measurement of cerebral oxidative glucose consumption in patients with type 1 diabetes mellitus and hypoglycemia unawareness using 13C nuclear magnetic resonance spectroscopy
AU - Henry, Pierre-Gilles
AU - Criego, Amy B.
AU - Kumar, Anjali
AU - Seaquist, Elizabeth R
N1 - Funding Information:
This work was supported by RO1NS38672 (PGH), RO1NS35192 (ERS), RO1DK62440 (ERS), P30NS057091, P41RR08679, and 5 MO1 RR0400.
PY - 2010/1
Y1 - 2010/1
N2 - The aim of the present study was to use 13C nuclear magnetic resonance (NMR) to measure the cerebral oxidative metabolic rate of glucose (CMRglc[ox]) in patients with diabetes and to compare these measurements with those collected from matched controls. We elected to study a group with type 1 diabetes mellitus and hypoglycemia unawareness because we had previously found such patients to have higher brain glucose concentrations than healthy volunteers under steady-state conditions. We sought to determine if this difference in steady-state brain concentrations could be explained by a difference in CMRglc(ox). Time courses of 13C label incorporation in brain amino acids were measured in occipital cortex during infusion of [1-13C]glucose. These time courses were fitted using a 1-compartment metabolic model to determine CMRglc(ox). Our results show that the tricarboxylic acid cycle (TCA) cycle rate (VTCA, which is twice CMRglc[ox]) in subjects with type 1 diabetes mellitus was not significantly different from that of healthy controls (0.84 ± 0.03 vs 0.79 ± 0.03 μmol/[g min], n = 5 in each group, mean ± SEM). We conclude that the changes in steady-state brain glucose concentrations that we observed in patients with type 1 diabetes mellitus in a previous study (J Neurosci Res. 2005;79:42-47) cannot be explained by changes in oxidative glucose consumption.
AB - The aim of the present study was to use 13C nuclear magnetic resonance (NMR) to measure the cerebral oxidative metabolic rate of glucose (CMRglc[ox]) in patients with diabetes and to compare these measurements with those collected from matched controls. We elected to study a group with type 1 diabetes mellitus and hypoglycemia unawareness because we had previously found such patients to have higher brain glucose concentrations than healthy volunteers under steady-state conditions. We sought to determine if this difference in steady-state brain concentrations could be explained by a difference in CMRglc(ox). Time courses of 13C label incorporation in brain amino acids were measured in occipital cortex during infusion of [1-13C]glucose. These time courses were fitted using a 1-compartment metabolic model to determine CMRglc(ox). Our results show that the tricarboxylic acid cycle (TCA) cycle rate (VTCA, which is twice CMRglc[ox]) in subjects with type 1 diabetes mellitus was not significantly different from that of healthy controls (0.84 ± 0.03 vs 0.79 ± 0.03 μmol/[g min], n = 5 in each group, mean ± SEM). We conclude that the changes in steady-state brain glucose concentrations that we observed in patients with type 1 diabetes mellitus in a previous study (J Neurosci Res. 2005;79:42-47) cannot be explained by changes in oxidative glucose consumption.
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U2 - 10.1016/j.metabol.2009.07.012
DO - 10.1016/j.metabol.2009.07.012
M3 - Article
C2 - 19766263
AN - SCOPUS:72049120933
SN - 0026-0495
VL - 59
SP - 100
EP - 106
JO - Metabolism: clinical and experimental
JF - Metabolism: clinical and experimental
IS - 1
ER -
