Measurement of bioaerosol filtration efficiency in an ASHRAE 52.2 filter test facility

Weihua Tang, M. A. Ramakrishnan, Sagar Goyal, Thomas Kuehn

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


A real-time monitor of bioaerosols, Fluorescence Laser Aerodynamic Particle Sizer (FLAPS), was evaluated for its ability to detect bioaerosols and measure bioaerosol filtration efficiency. Biological spores (Bacillus subtilis and Cladosporium sphaerospermum) were aerosolized and injected into a wind tunnel based on ASHRAE Standard 52.2. The sensitivity of the FLAPS was adjusted by the photomultiplier tube (PMT) gain to distinguish between bioaerosols and non-biological aerosols such as polystyrene latex and potassium chloride particles. Suspensions of biological spores were carefully prepared to remove interference with culture media. Aerosol concentrations upstream and downstream of a clean synthetic media filter (MERV-14) with electrostatic charge were monitored by FLAPS and by an Andersen impactor. The calculated filtration efficiency from the measurement of FLAPS was 96% for B. Subtilis and 100% for C. sphaerospermum. These results agree with those obtained with the Andersen impactor (91% for B. subtilis and 99.5% for C. sphaerospermum).

Original languageEnglish (US)
Pages (from-to)40-44
Number of pages5
Issue number1
StatePublished - Jan 1 2007

Fingerprint Dive into the research topics of 'Measurement of bioaerosol filtration efficiency in an ASHRAE 52.2 filter test facility'. Together they form a unique fingerprint.

Cite this