Mcm10: A dynamic scaffold at eukaryotic replication forks

Research output: Contribution to journalReview article

12 Citations (Scopus)

Abstract

To complete the duplication of large genomes efficiently, mechanisms have evolved that coordinate DNA unwinding with DNA synthesis and provide quality control measures prior to cell division. Minichromosome maintenance protein 10 (Mcm10) is a conserved component of the eukaryotic replisome that contributes to this process in multiple ways. Mcm10 promotes the initiation of DNA replication through direct interactions with the cell division cycle 45 (Cdc45)-minichromosome maintenance complex proteins 2-7 (Mcm2-7)-go-ichi-ni-san GINS complex proteins, as well as single- and double-stranded DNA. After origin firing, Mcm10 controls replication fork stability to support elongation, primarily facilitating Okazaki fragment synthesis through recruitment of DNA polymerase-α and proliferating cell nuclear antigen. Based on its multivalent properties, Mcm10 serves as an essential scaffold to promote DNA replication and guard against replication stress. Under pathological conditions, Mcm10 is often dysregulated. Genetic amplification and/or overexpression of MCM10 are common in cancer, and can serve as a strong prognostic marker of poor survival. These findings are compatible with a heightened requirement for Mcm10 in transformed cells to overcome limitations for DNA replication dictated by altered cell cycle control. In this review, we highlight advances in our understanding of when, where and how Mcm10 functions within the replisome to protect against barriers that cause incomplete replication.

Original languageEnglish (US)
Article number73
JournalGenes
Volume8
Issue number2
DOIs
StatePublished - Feb 17 2017

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Minichromosome Maintenance Proteins
DNA Replication
DNA
Single-Stranded DNA
Proliferating Cell Nuclear Antigen
DNA-Directed DNA Polymerase
Cell Cycle Checkpoints
Quality Control
Cell Division
Cell Cycle
Genome

Keywords

  • CMG helicase
  • DNA replication
  • Genome stability
  • Mcm10
  • Origin activation
  • Replication elongation
  • Replication initiation

Cite this

Mcm10 : A dynamic scaffold at eukaryotic replication forks. / Baxley, Ryan M.; Bielinsky, Anja Katrin.

In: Genes, Vol. 8, No. 2, 73, 17.02.2017.

Research output: Contribution to journalReview article

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