Mccarey-kaufman (MK) Organ Culture And MK Medium-Shifted Corneas

J. Daniel Nelson, Dan B. Lange, Richard L. Lindstrom, Donald J. Doughman, Diane L. Hatchell

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Paired human donor corneas were studied to determine the effects of temperature, osmolarity, and media shifts on the endothelium of corneas stored in McCarey-Kaufman (MK) medium initially and then shifted to standard organ culture medium. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) demonstrated intact corneal endothelium in six of seven human control corneas stored in MK medium prior to fixation. Intact corneal endothelium was similarly demonstrated by specular microscopy, SEM, and TEM in seven of seven “MK-shifted” corneas. These data suggest that MK-stored donor corneas may be transferred into an organ culture system at 34 °C for prolonged storage with maintenance of corneal endothelial cell viability.

Original languageEnglish (US)
Pages (from-to)308-311
Number of pages4
JournalArchives of Ophthalmology
Volume102
Issue number2
DOIs
StatePublished - Jan 1 1984

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Organ Culture Techniques
Cornea
Corneal Endothelium
Transmission Electron Microscopy
Electron Scanning Microscopy
Osmolar Concentration
Endothelium
Culture Media
Microscopy
Cell Survival
Endothelial Cells
Maintenance
McCarey-Kaufman medium
Temperature

Cite this

Nelson, J. D., Lange, D. B., Lindstrom, R. L., Doughman, D. J., & Hatchell, D. L. (1984). Mccarey-kaufman (MK) Organ Culture And MK Medium-Shifted Corneas. Archives of Ophthalmology, 102(2), 308-311. https://doi.org/10.1001/archopht.1984.01040030252036

Mccarey-kaufman (MK) Organ Culture And MK Medium-Shifted Corneas. / Nelson, J. Daniel; Lange, Dan B.; Lindstrom, Richard L.; Doughman, Donald J.; Hatchell, Diane L.

In: Archives of Ophthalmology, Vol. 102, No. 2, 01.01.1984, p. 308-311.

Research output: Contribution to journalArticle

Nelson, JD, Lange, DB, Lindstrom, RL, Doughman, DJ & Hatchell, DL 1984, 'Mccarey-kaufman (MK) Organ Culture And MK Medium-Shifted Corneas', Archives of Ophthalmology, vol. 102, no. 2, pp. 308-311. https://doi.org/10.1001/archopht.1984.01040030252036
Nelson, J. Daniel ; Lange, Dan B. ; Lindstrom, Richard L. ; Doughman, Donald J. ; Hatchell, Diane L. / Mccarey-kaufman (MK) Organ Culture And MK Medium-Shifted Corneas. In: Archives of Ophthalmology. 1984 ; Vol. 102, No. 2. pp. 308-311.
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