Matrix-matrix interaction of cartilage oligomeric matrix protein and fibronectin

Paul E. Di Cesare, Frank S. Chen, Matthias Moergelin, Cathy S. Carlson, Michael P. Leslie, Roberto Perris, Carrie Fang

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102 Scopus citations


Recent work indicates that cartilage oligomeric matrix protein (COMP) plays an important role in extracellular matrix assembly and matrix-matrix protein interactions. In order to identify the proteins in extracellular matrix that interact with COMP, we used an ELISA-based solid-phase binding assay, which revealed a specific, high-affinity interaction between COMP and fibronectin. This interaction is concentration-dependent and saturable, and appears to occur under physiologically relevant conditions. Electron microscopy after negative staining and fragment binding analysis using the solid-phase assay revealed a predominant binding site for the COMP C-terminal globular domain to a molecular domain approximately 14 nm from the N-terminal domain of fibronectin, which can be inhibited by the presence of a polyclonal antibody specific for the C-terminal heptadecapeptide of COMP. This interaction is further demonstrated in vivo by colocalization of both COMP and fibronectin in the chondrocyte pericellular matrix by laser confocal microscopy of chondrocytes grown in agarose culture, and by appositional and colocalization of these proteins in the growth plate of primates by immunohistochemistry.

Original languageEnglish (US)
Pages (from-to)461-470
Number of pages10
JournalMatrix Biology
Issue number5
StatePublished - Aug 2002

Bibliographical note

Funding Information:
This study was made possible by funding from the National Institutes of Health (NIH RO1 AR45612-01A2 and NIH RR14099), Orthopedic Education Research Foundation, Fondo Sanitario Nazionale, Italian Ministry of Health, Arthritis Foundation, The Swedish Medical Research Council, and Human Growth Foundation. The authors thank Ms Maria Teresa Mucignat, Ms Yi Lou, and Mr Eric Chang for technical assistance and Dr Vishwas Ganu for his generous gift of polyclonal antibody H781.


  • Cartilage oligomeric matrix protein (COMP)
  • Fibronectin
  • Matrix-matrix protein interaction


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