Mast cell-derived mediators protect against oxygen-glucose deprivation-induced injury in PC12 cells and neurons

Weiwei Hu, Yanyin Fan, Yao Shen, Yuzhe Yang, Haibin Dai, Qiuli Fu, Zhong Chen

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Recent reports and our previous study suggest that mast cells play a crucial role in the pathological processes that follow cerebral ischemia. In this study, the effect of mast cells on neuron injury after cerebral ischemia was determined by adding in vitro ischemia-induced supernatant from mast cells to neurons and PC12 cells under the same conditions (oxygen-glucose deprivation, OGD). The degree of cell injury was evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-dipheny-ltetrazolium bromide (MTT) assay. Mast cell-derived supernatant protected against OGD-induced injury of PC12 cells and neurons, and this protection was reversed by a histamine H1 antagonist and by anti-histamine serum, but not by an H2 antagonist. However, histamine and nerve growth factor (NGF) added separately or together did not have protective effects against OGD-induced injury. These results indicate that mast cell-derived protection during in vitro ischemia is histamine-dependent, and involves cooperation with other mediators, but not NGF.

Original languageEnglish (US)
Pages (from-to)35-40
Number of pages6
JournalNeuroscience Letters
Volume423
Issue number1
DOIs
StatePublished - Aug 9 2007
Externally publishedYes

Bibliographical note

Funding Information:
This project was supported by the National Natural Science Foundation of China (30572176, 30600757), the National Basic Research of China (2003CB515400), and partly by the Zhejiang Provincial Natural Science Foundation of China (R303779). We are very grateful to Dr. Iain C. Bruce (University of Hong Kong) for language polishing.

Keywords

  • Histamine
  • Mast cell
  • Neurons
  • Oxygen-glucose deprivation (OGD)
  • PC12 cells

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