Abstract
Freeze substitution microscopy and a differential scanning calorimeter (DSC) were used to quantify freeze-induced dehydration within Dunning AT-1 rat prostate tumor tissue. Stereological analysis of histological tumor sections obtained the initial cellular, interstitial, and vascular volumes of the tissue. A model of water transport was fitted to the DSC data at 5, 10 and 20 °C/min to obtain the combined best fit membrane permeability parameters of the embedded AT-1 tumor cells, assuming either a Krogh cylinder geometry or a spherical cell geometry. Numerical simulations were performed to generate conservative estimates of intracellular ice volume in the tumor tissue at various cooling rates typical of those experienced during cryosurgery.
Original language | English (US) |
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Pages (from-to) | 639-654 |
Number of pages | 16 |
Journal | AIChE Journal |
Volume | 45 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1999 |