Mass cultivation of microalgae on animal wastewater: A sequential two-stage cultivation process for energy crop and omega-3-rich animal feed production

Wenguang Zhou, Bing Hu, Yecong Li, Min Min, Michael Mohr, Zhenyi Du, Paul Chen, Roger Ruan

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

In this study, 97 microalgal strains purchased from algae bank and 50 microalgal strains isolated from local waters in Minnesota were screened for their adaptability growing on a 20-fold diluted digested swine manure wastewater (DSMW). A pool of candidate strains well adapted to the DSMW was established through a high-throughput screening process. Two top-performing facultative heterotrophic strains with high growth rate (0.536 day-1 for UMN 271 and 0.433 day-1 for UMN 231) and one strain with high omega-3 unsaturated fatty acid (EPA, 3.75 % of total fatty acids for UMN 231) were selected. Subsequently, a sequential two-stage mixo-photoautotrophic culture strategy was developed for biofuel and animal feed production as well as simultaneous swine wastewater treatment using above two strains. The maximal biomass concentration and lipid content at the first and second stages reached 2.03 g/L and 23.0 %, and 0.83 g/L and 19.0 % for UMN 271 and UMN 231, respectively. The maximal nutrient removals for total phosphorus and ammonia after second-stage cultivation were 100 and 89.46 %, respectively. The experiments showed that this sequential two-stage cultivation process has great potential for economically viable and environmentally friendly production of both renewable biofuel and high-value animal feed and at the same time for animal wastewater treatment.

Original languageEnglish (US)
Pages (from-to)348-363
Number of pages16
JournalApplied Biochemistry and Biotechnology
Volume168
Issue number2
DOIs
StatePublished - Sep 2012

Bibliographical note

Funding Information:
Acknowledgments The study was partially supported by grants from the University of Minnesota Initiative for Renewable Energy and the Environment, Metropolitan Council Environmental Services, Xcel Energy, and the Legislative-Citizen Commission on Minnesota Resources. The authors are also grateful to Blanca C. Martinez for providing help in the labs.

Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.

Keywords

  • Animal feed
  • Energy crop
  • Facultative heterotrophic microalgae
  • Mixo-photoautotrophic cultivation
  • Omega-3 fatty acid
  • Swine manure wastewater

Fingerprint

Dive into the research topics of 'Mass cultivation of microalgae on animal wastewater: A sequential two-stage cultivation process for energy crop and omega-3-rich animal feed production'. Together they form a unique fingerprint.

Cite this