Marker Assisted Selection in Sorghum Using KASP Assay for the Detection of Single Nucleotide Polymorphism/Insertion Deletion

Gloria Burow, Ratan Chopra, Halee Hughes, Zhanguo Xin, John Burke

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations


Marker assisted selection (MAS), an advance tool in plant breeding that allows accurate and efficient introgression of important agronomic trait(s) from a germplasm source to desired elite lines, has been applied to sorghum recently. Here, we report the methods for the deployment of MAS for trait introgression using endpoint genotyping technology for single nucleotide polymorphism (SNP)/insertion deletion (InDel) coupled with an application of KASP (Kompetitive Allele Specific Polymerase Chain Reaction [PCR]) chemistry allowing for the selection of parents for generational advancement without going through the laborious and time consuming phenotypic selection and additional generations for selection of desired individuals. This MAS-SNP marker assisted backcrossing scheme can be applied to accurately select heterozygotes for use as an allele donor parent in each backcross generation, thus expediting the backcrossing scheme and resulting in time savings of 3 years compared to conventional methods of introgression practiced in sorghum breeding and improvement.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Number of pages10
StatePublished - Jan 17 2019

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.


  • Cluster analysis
  • Endpoint genotyping
  • Kompetitive Allele Specific PCR(KASP)
  • Marker assisted selection (MAS)
  • Single nucleotide polymorphism (SNP)
  • Plant Breeding/methods
  • Sorghum/genetics
  • Genotype
  • Genetic Markers/genetics
  • Gene Editing/methods
  • Phenotype
  • Polymorphism, Single Nucleotide/genetics
  • Quantitative Trait Loci/genetics
  • Alleles
  • INDEL Mutation/genetics

PubMed: MeSH publication types

  • Research Support, Non-U.S. Gov't
  • Journal Article


Dive into the research topics of 'Marker Assisted Selection in Sorghum Using KASP Assay for the Detection of Single Nucleotide Polymorphism/Insertion Deletion'. Together they form a unique fingerprint.

Cite this