Mapping tissue oxygen in vivo by photoacoustic lifetime imaging

Qi Shao, Ekaterina Morgounova, Jeung Hwan Choi, Chunlan Jiang, John Bischof, Shai Ashkenazi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Oxygen plays a key role in the energy metabolism of living organisms. Any imbalance in the oxygen levels will affect the metabolic homeostasis and lead to pathophysiological diseases. Hypoxia, a status of low tissue oxygen, is a key factor in tumor biology as it is highly prominent in tumor tissues. However, clinical tools for assessing tissue oxygenation are limited. The gold standard is polarographic needle electrode which is invasive and not capable of mapping (imaging) the oxygen content in tissue. We applied the method of photoacoustic lifetime imaging (PALI) of oxygen-sensitive dye to small animal tissue hypoxia research. PALI is new technology for direct, non-invasive imaging of oxygen. The technique is based on mapping the oxygen-dependent transient optical absorption of Methylene Blue (MB) by pump-probe photoacoustic imaging. Our studies show the feasibility of imaging of dissolved oxygen distribution in phantoms. In vivo experiments demonstrate that the hypoxia region is consistent with the site of subcutaneously xenografted prostate tumor in mice with adequate spatial resolution and penetration depth.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2013
DOIs
StatePublished - May 28 2013
EventPhotons Plus Ultrasound: Imaging and Sensing 2013 - San Francisco, CA, United States
Duration: Feb 3 2013Feb 5 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8581
ISSN (Print)1605-7422

Conference

ConferencePhotons Plus Ultrasound: Imaging and Sensing 2013
CountryUnited States
CitySan Francisco, CA
Period2/3/132/5/13

Keywords

  • hypoxia
  • lifetime imaging
  • photoacoustic
  • tissue oxygen
  • tumor

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