Mapping the phase diagram of single DNA molecule force-induced melting in the presence of ethidium

Ioana D. Vladescu, Micah J. Mccauley, Ioulia Rouzina, Mark C. Williams

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

When a single DNA molecule is stretched beyond its normal contour length, a force-induced melting transition is observed. Ethidium binding increases the DNA contour length, decreases the elongation upon melting, and increases the DNA melting force in a manner that is consistent with the ethidium-induced changes in duplex DNA stability known from thermal melting studies. The DNA stretching curves map out a phase diagram and critical point in the force-extension- ethidium concentration space. Intercalation occurs between alternate base pairs at low forces and between every base pair at high forces.

Original languageEnglish (US)
Article number158102
JournalPhysical review letters
Volume95
Issue number15
DOIs
StatePublished - Oct 7 2005

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