Mapping of beta-tubulin genomic sequences in hexaploid oat (Arena sativa L.)

N. Mendu, H. Rines, C. D. Silflow

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The allohexaploid nature of Avena sativa L. (2n=6x=42) and the availability of aneuploid lines was exploited in designing a strategy for mapping beta-tubulin sequences in the oat genome. Evidence for a minimum of eight beta-tubulin genes was obtained by Southern-blot analysis. Three betatubulin sequences were localized to chromosomes using DNA from monosomic and nullisomic lines in the variety Sun II. One sequence was localized to the chromosome missing in nullisome I. Two other sequences were mapped to satellite chromosome 2, the chromosome that is missing in nullisome VIII and to which one ribosomal RNA gene cluster had previously been mapped. Restriction fragments carrying these two beta-tubulin genomic sequences and the cluster of ribosomal RNA sequences were missing in DNA from nullisomics VIII, IX and X, suggesting that all three nullisome classes are deficient for an identical chromosomal segment that includes these three loci. This study demonstrates how molecular analyses can be used to characterize aneuploid stocks and to better define their genetic constitution.

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalTheoretical and Applied Genetics
Volume86
Issue number1
DOIs
StatePublished - Mar 1 1993

Keywords

  • Avena sativa L.
  • Beta-tubulin
  • Mapping
  • Monosomic
  • Nullisomic

Fingerprint

Dive into the research topics of 'Mapping of beta-tubulin genomic sequences in hexaploid oat (Arena sativa L.)'. Together they form a unique fingerprint.

Cite this